Abstract

The evidence obtained in prior years from this project suggests that prostaglandins are likely candidates for mediators of at least some of the actions of cannabinoids. The primary goal of the present proposal is to further pursue this hypothesis with emphasis on the psychotropic actions of Delta1-THC. An additional goal is to show that the effects of cannabionoids on arachidonic acid metabolism in cell culture systems is a useful model for studying the underlying molecular events involved in cannabinoid action. Thus, this project is part of the overall effort in our laboratory which is to study the mechanism of action of cannabinoids. Experiments will be done to further describe the effects of cannabinoids on prostaglandin synthesis both in vivo in mice and in vitro using a variety of cell lines. Structure-activity profiles will be obtained and phenomena such as tolerance and drug interactions will be studied in these model systems. These experiments will be designed to complement our more general interest in the biochemical events surrounding cannabinoid actions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Project (R01)
Project #
5R01DA002043-11
Application #
3207095
Study Section
(SRC)
Project Start
1977-09-01
Project End
1988-07-31
Budget Start
1987-08-01
Budget End
1988-07-31
Support Year
11
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Massachusetts Medical School Worcester
Department
Type
Schools of Medicine
DUNS #
660735098
City
Worcester
State
MA
Country
United States
Zip Code
01655

  Publications

Audette, C A; Burstein, S H; Doyle, S A et al. (1991) G-protein mediation of cannabinoid-induced phospholipase activation. Pharmacol Biochem Behav 40:559-63
Burstein, S (1991) Cannabinoid induced changes in eicosanoid synthesis by mouse peritoneal cells. Adv Exp Med Biol 288:107-12
Perez-Reyes, M; Burstein, S H; White, W R et al. (1991) Antagonism of marihuana effects by indomethacin in humans. Life Sci 48:507-15
Doyle, S A; Burstein, S H; Dewey, W L et al. (1990) Further studies on the antinociceptive effects of delta 6-THC-7-oic acid. Agents Actions 31:157-63
Audette, C A; Burstein, S (1990) Inhibition of leukocyte adhesion by the in vivo and in vitro administration of cannabinoids. Life Sci 47:753-9
Burstein, S H; Hull, K; Hunter, S A et al. (1989) Immunization against prostaglandins reduces delta 1-tetrahydrocannabinol-induced catalepsy in mice. Mol Pharmacol 35:6-9
Burstein, S H; Audette, C A; Doyle, S A et al. (1989) Antagonism to the actions of platelet activating factor by a nonpsychoactive cannabinoid. J Pharmacol Exp Ther 251:531-5
Burstein, S H; Hull, K; Hunter, S A et al. (1988) Cannabinoids and pain responses: a possible role for prostaglandins. FASEB J 2:3022-6
Burstein, S H (1987) Inhibitory and stimulatory effects of cannabinoids on eicosanoid synthesis. NIDA Res Monogr 79:158-72
Burstein, S; Hunter, S A; Latham, V et al. (1987) A major metabolite of delta 1-tetrahydrocannabinol reduces its cataleptic effect in mice. Experientia 43:402-3

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