Abstract

We will continue our research into the effect of phencyclidine (PCP) on identified ctecholaminergic neuronal pathways in rat central nervous system. Using electrophysiological and electrochemical techniques, we have characterized PCP as an indirect dopamineergic agonist in caudate and as an indirect noradrenergic agonist in cerebellum and hippocampus. In the present application, we will extend this work to consideration of dopaminergic pathways arising in the ventral tegmental area, which project to nucleus accumbens and frontal cortex. We will study these structures in intact anesthetized animals, as well as isolated neuronal circuits transplanted in oculo and in situ. We will extend our pharmacological studies to include interaction between sigma opiates and PCP. We will also study the effects of repeated administration of PCP over several weeks on electrochemical and electrophysiological parameters of catecholaminergic neurotransmission. Finally, we will chracterize the effects of PCP in unanesthetized freely-moving animals and then, in this preparation, study catecholaminergic neurotransmission during PCP self-administration.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Project (R01)
Project #
5R01DA002429-09
Application #
3207328
Study Section
(DABA)
Project Start
1979-08-01
Project End
1988-12-31
Budget Start
1987-08-01
Budget End
1988-12-31
Support Year
9
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Colorado Denver
Department
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045

  Publications

Kim, M; Bickford, P C (1992) Electrophysiological effects of phencyclidine and the sigma agonist ditolylguanidine in the cerebellum of the rat. Neuropharmacology 31:77-83
Miller, C L; Bickford, P C; Luntz-Leybman, V et al. (1992) Phencyclidine and auditory sensory gating in the hippocampus of the rat. Neuropharmacology 31:1041-8
Kao, M C; Lee, H K; Chai, C Y et al. (1991) NMDA antagonists attenuate hypertension induced by carotid clamping in the rostral ventrolateral medulla of rats. Brain Res 549:83-9
Wang, Y; Lee, H K (1991) Electrophysiological interactions between NMDA and phencyclidine/sigma receptor agonists and antagonists in Purkinje neurons in the cerebellum of the rat. Neuropharmacology 30:985-94
Gratton, A; Hoffer, B J; Gerhardt, G A (1989) In vivo electrochemical studies of monoamine release in the medial prefrontal cortex of the rat. Neuroscience 29:57-64
Gerhardt, G A; Drebing, C J; Stephen, C et al. (1989) Direct determination of unconjugated HVA in human plasma filtrates by HPLC coupled with dual-electrode coulometric electrochemical detection. Biomed Chromatogr 3:105-9
de la Garza, R; Freedman, R; Hoffer, B J (1989) Kappa-bungarotoxin blockade of nicotine electrophysiological actions in cerebellar Purkinje neurons. Neurosci Lett 99:95-100
de la Garza, R; Freedman, R; Hoffer, J (1989) Nicotine-induced inhibition of cerebellar Purkinje neurons: specific actions of nicotine and selective blockade by mecamylamine. Neuropharmacology 28:495-501
Gratton, A; Hoffer, B J; Gerhardt, G A et al. (1988) Potentiation of morphine-elicited circling by dopaminergic uptake blockade. Pharmacol Biochem Behav 30:1077-9
Gratton, A; Hoffer, B J; Gerhardt, G A (1988) Effects of electrical stimulation of brain reward sites on release of dopamine in rat: an in vivo electrochemical study. Brain Res Bull 21:319-24

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