Nicotine is a major addictive drug that recruits the endogenous opioid system for some of its addictive effects. We have validated sophisticated behavioral models for the evaluation of genetic factors that contribute to nicotine addiction in mice. These models will be used in conventional and tissue-specific opioid receptor and opioid peptide knockout mice to dissect the participation of opioid mechanisms in nicotine addiction and to identify genes related to nicotine consumption downstream of the opioid system.
The specific aims will be:
Specific Aim 1. To evaluate the involvement of opioid receptors and endogenous opioid peptides in the different components of nicotine addiction.
Specific Aim 2. To validate the behavioral changes observed in Aim 1 and clarify neural sites involved by using conditional tissue-specific knockout of opioid receptor and peptide genes.
Specific Aim 3. To identify genes regulated by nicotine downstream the opioid system. The potential impact of results obtained in this research project is of importance since nicotine addiction constitutes a major problem of public health in the world. These results will help to identify potential targets for the development of novel therapeutic strategies against nicotine addiction. Therefore, this project could provide a major progress in the management of nicotine dependence, and could have repercussions to improve the quality of live in the societies widely affected by the health and social-economic problems associated with nicotine addiction. Finally, the results and experimental strategies of this project would be also of interest for the study of other addictive processes, considering that the opioid system represents a common substrate to the addictive properties of most drugs of abuse.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Project (R01)
Project #
5R01DA016768-02
Application #
6952233
Study Section
Biobehavioral Regulation, Learning and Ethology Study Section (BRLE)
Program Officer
Volman, Susan
Project Start
2004-09-01
Project End
2009-07-31
Budget Start
2005-09-30
Budget End
2006-07-31
Support Year
2
Fiscal Year
2005
Total Cost
$308,000
Indirect Cost
Name
Pompeu Fabra University
Department
Type
DUNS #
469951313
City
Barcelona
State
Country
Spain
Zip Code
08002
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Goeldner, Celia; Lutz, Pierre-Eric; Darcq, Emmanuel et al. (2011) Impaired emotional-like behavior and serotonergic function during protracted abstinence from chronic morphine. Biol Psychiatry 69:236-44
Le Merrer, Julie; Plaza-Zabala, Ainhoa; Del Boca, Carolina et al. (2011) Deletion of the ? opioid receptor gene impairs place conditioning but preserves morphine reinforcement. Biol Psychiatry 69:700-3

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