Abstract

In this series of studies sophisticated track tracing experiments at a light and electron microscopic level will be used to examine development/plasticity during the first two months after onset of hearing in the ferret. Proposed studies will focus on altered laterality of afferent projections from and to brainstem auditory nuclei. These studies will examine if exuberant proliferation with subsequent refinement of lateral superior olivary nucleus projections and/or refinement of synaptic circuitry occurs in the IC. This first series of studies will serve as a baseline for studies of monaural deprivation. Deprivation studies will help determine the possible role of competition between pathways excited by each ear in developmental plasticity. Both monaural and binaural deprivation using ear plugging will be used to alter the auditory experience during the first two months after onset of hearing in the ferret. Results should lead to a better understanding of the influence of experience on the development of these binaural sensory pathways.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC000813-08
Application #
6175020
Study Section
Special Emphasis Panel (ZRG1-SEN (01))
Program Officer
Luethke, Lynn E
Project Start
1990-09-30
Project End
2003-03-31
Budget Start
2000-04-01
Budget End
2001-03-31
Support Year
8
Fiscal Year
2000
Total Cost
$227,243
Indirect Cost

  Institution

Name
Wake Forest University Health Sciences
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
937727907
City
Winston-Salem
State
NC
Country
United States
Zip Code
27157

  Publications

Alvarado, Juan Carlos; Fuentes-Santamaria, Veronica; Henkel, Craig K (2009) Rapid modifications in calretinin immunostaining in the deep layers of the superior colliculus after unilateral cochlear ablation. Hear Res 247:78-86
Fuentes-Santamaria, V; Alvarado, J Carlos; Henkel, C K et al. (2007) Cochlear ablation in adult ferrets results in changes in insulin-like growth factor-1 and synaptophysin immunostaining in the cochlear nucleus. Neuroscience 148:1033-47
Alvarado, Juan Carlos; Fuentes-Santamaria, Veronica; Franklin, Samuel R et al. (2007) Synaptophysin and insulin-like growth factor-1 immunostaining in the central nucleus of the inferior colliculus in adult ferrets following unilateral cochlear removal: a densitometric analysis. Synapse 61:288-302
Varga, R; Avenarius, M R; Kelley, P M et al. (2006) OTOF mutations revealed by genetic analysis of hearing loss families including a potential temperature sensitive auditory neuropathy allele. J Med Genet 43:576-81
Alvarado, J C; Fuentes-Santamaria, V; Franklin, S R et al. (2005) Unilateral cochlear ablation in adult ferrets results in upregulation in calretinin immunostaining in the central nucleus of the inferior colliculus. Neuroscience 136:957-69
Fuentes-Santamaria, Veronica; Alvarado, Juan Carlos; Taylor, Anna R et al. (2005) Quantitative changes in calretinin immunostaining in the cochlear nuclei after unilateral cochlear removal in young ferrets. J Comp Neurol 483:458-75
Henkel, C K; Gabriele, M L; McHaffie, J G (2005) Quantitative assessment of developing afferent patterns in the cat inferior colliculus revealed with calbindin immunohistochemistry and tract tracing methods. Neuroscience 136:945-55
Alvarado, Juan Carlos; Fuentes-Santamaria, Veronica; Henkel, Craig K et al. (2004) Alterations in calretinin immunostaining in the ferret superior olivary complex after cochlear ablation. J Comp Neurol 470:63-79
Varga, R; Kelley, P M; Keats, B J et al. (2003) Non-syndromic recessive auditory neuropathy is the result of mutations in the otoferlin (OTOF) gene. J Med Genet 40:45-50
Huang, Qing-Yang; Shen, Hui; Deng, Hong-Yi et al. (2003) Linkage and association of the CA repeat polymorphism of the IL6 gene, obesity-related phenotypes, and bone mineral density (BMD) in two independent Caucasian populations. J Hum Genet 48:430-7

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