S Pneumoniae Adherence and Otitis Media Pathogenesis

Demaria, Thomas F.

Abstract

The specific aims of this proposal are to (i) assess the adherence capability of the Streptococcus pneumoniae (Spn) serotypes 3,6,14,18,19 and 23 which are capsular types specifically associated with episodes of OM and define the mechanisms involved in this interaction using both in vitro and in vivo models of adherence and adherence inhibition to better understand this critical host-bacteria cell interrelationship. Specifically, the role of lectin receptors of Spn in adherence and invasion will be investigated; (ii) assess the role of Spn neuraminidase in adherence and the pathogenesis of OM; (iii) assess the effect of adenovirus and influenza A virus on Spn adherence, colonization, and OM.
These aims delineate steps that culminate in otitis media.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute on Deafness and Other Communication Disorders (NIDCD)
• Type: Research Project (R01)
• Project #: 5R01DC003105-04
• Application #: 6043386
• Study Section: Hearing Research Study Section (HAR)
• Project Start: 1996-08-01
• Project End: 2000-07-31
• Budget Start: 1999-08-01
• Budget End: 2000-07-31
• Support Year: 4
• Fiscal Year: 1999

Institution

• Name: Ohio State University
• Department: Otolaryngology
• Type: Schools of Medicine
• DUNS #: 098987217
• City: Columbus
• State: OH
• Country: United States
• Zip Code: 43210

Publications

Chen, Shu; Paterson, Gavin K; Tong, Hua Hua et al. (2005) Sortase A contributes to pneumococcal nasopharyngeal colonization in the chinchilla model. FEMS Microbiol Lett 253:151-4

Tong, H H; Li, D; Chen, S et al. (2005) Immunization with recombinant Streptococcus pneumoniae neuraminidase NanA protects chinchillas against nasopharyngeal colonization. Infect Immun 73:7775-8

Long, J P; Tong, H H; DeMaria, T F (2004) Immunization with native or recombinant Streptococcus pneumoniae neuraminidase affords protection in the chinchilla otitis media model. Infect Immun 72:4309-13

Tong, Hua Hua; Long, James P; Li, Daneng et al. (2004) Alteration of gene expression in human middle ear epithelial cells induced by influenza A virus and its implication for the pathogenesis of otitis media. Microb Pathog 37:193-204

Tong, H H; Long, J P; Shannon, P A et al. (2003) Expression of cytokine and chemokine genes by human middle ear epithelial cells induced by influenza A virus and Streptococcus pneumoniae opacity variants. Infect Immun 71:4289-96

Long, J P; Tong, H H; Shannon, P A et al. (2003) Differential expression of cytokine genes and inducible nitric oxide synthase induced by opacity phenotype variants of Streptococcus pneumoniae during acute otitis media in the rat. Infect Immun 71:5531-40

Tong, H H; Grants, I; Liu, X et al. (2002) Comparison of alteration of cell surface carbohydrates of the chinchilla tubotympanum and colonial opacity phenotype of Streptococcus pneumoniae during experimental pneumococcal otitis media with or without an antecedent influenza A virus infection. Infect Immun 70:4292-301

Tong, Hua Hua; Liu, Xia; Chen, Yiping et al. (2002) Effect of neuraminidase on receptor-mediated adherence of Streptococcus pneumoniae to chinchilla tracheal epithelium. Acta Otolaryngol 122:413-9

Tong, H H; Weiser, J N; James, M A et al. (2001) Effect of influenza A virus infection on nasopharyngeal colonization and otitis media induced by transparent or opaque phenotype variants of Streptococcus pneumoniae in the chinchilla model. Infect Immun 69:602-6

Tong, H H; James, M; Grants, I et al. (2001) Comparison of structural changes of cell surface carbohydrates in the eustachian tube epithelium of chinchillas infected with a Streptococcus pneumoniae neuraminidase-deficient mutant or its isogenic parent strain. Microb Pathog 31:309-17