Mucoid otitis media is characterized by thick and visco-elastic effusions in the middle ear that reflect high concentrations of mucins. It is one of the most common otologic diseases in children and can ultimately scar the middle ear and lead to persistent hearing loss and communication problems. There is increasing evidence that mucoid otitis media results from the acute forms of otitis media along a continuum. The pathogenesis of this continuum is not well understood, but most likely involves the alteration of mucin metabolism. We proposed to investigate the pathogenic mechanisms of mucoid otitis media focusing on the alteration of mucus cell metaplasia and mucin gene expression as the key events leading to this transition. We will identify factors that modulate gene expression and mucus cell proliferation using primary culture or rat middle ear epithelial cells and experimental models of otitis media. Identifying the triggers that create the transition of acute to chronic otitis media may enable us to develop a noel therapeutic strategy to interrupt these triggers and stop the pathogenesis of this continuum.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
3R01DC003433-02S1
Application #
6356682
Study Section
Special Emphasis Panel (ZRG1 (01))
Program Officer
Johnson, Thomas E
Project Start
1998-12-01
Project End
2003-11-30
Budget Start
2000-09-28
Budget End
2000-11-30
Support Year
2
Fiscal Year
2000
Total Cost
$50,000
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Pediatrics
Type
Schools of Medicine
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
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Ozeki, Masashi; Schlentz, Eileen P; Lin, Jizhen (2005) Characterization of inhibitor of differentiation (Id3) gene expression in the developing cochlear tissue of rats. Acta Otolaryngol 125:244-9
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Tsuchiya, Katsuyuki; Kim, Youngki; Ondrey, Frank G et al. (2005) Characterization of a temperature-sensitive mouse middle ear epithelial cell line. Acta Otolaryngol 125:823-9
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Lee, Yun-Woo; Ozeki, Masashi; Juhn, Steven K et al. (2004) Expression of platelet-derived growth factor in the developing cochlea of rats. Acta Otolaryngol 124:558-62
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Lin, Jizhen; Tsuboi, Yasuhiro; Pan, Wei et al. (2002) Analysis by cDNA microarrays of altered gene expression in middle ears of rats following pneumococcal infection. Int J Pediatr Otorhinolaryngol 65:203-11

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