The planned studies are designed to develop a rapid means of phenotyping mice according to the functional status of their peripheral ears. The initial experiments in Years 1-1.5 focus on developing a protocol using distortion-product otoacoustic emissions (DPOAEs) that is suitable for screening large numbers of mice for cochlear abnormalities. Toward this goal, 2f1-f2 DPOAEs will be obtained at three levels in the form of DPgrams that will test at 10 points per octave over a frequency extent that ranges from a geometric mean of 5.6 to 48.5 kHz (f2=60 kHz). These measures will be recorded from both ears of mice representing 40 unique strains, with n=12 mice from each strain. In addition, such DPOAE-screening data will be obtained at two ages in the same mice, ie, at 2 and 5 mo of age. The database created by these data will be analyzed in order to determine the 5th percentile of DPOAE levels in normal CBA-type mice and the 95th percentile in mice, such as the C57, with impaired cochlear function. The percentile data will be used to devise a standardized chart upon which data for individual mice undergoing mutagenesis can be plotted in order to determine if cochlear function is normal or impaired. In association with these experiments, a commercial instrument will be developed with a leading manufacturer of auditory-research equipment, which can be made available in the marketplace so that the scientific community will have access to the ability to determine the status of cochlear function in unknown mice. The second stage of the project, to be conducted in another group of mice from Years 1.5 to 3 of the study, is aimed at establishing a similar database for these same 40 mouse strains based on their susceptibility to noise-induced hearing loss. To accomplish this goal, following the acquisition of baseline DP-grams, at 3 mo mice will be exposed to a standard octave band noise known to cause permanent decrements in DPOAE levels, and then re-tested 2 wks later in order to document the effects of noise overexposure on cochlear function. Percentiles from these exposed mouse groups will form the basis of another standardized chart for determining the susceptibility of individual mice to acoustic overstimulation. Together, both projects will establish the usefulness of DPOAE testing for the purpose of developing simple and rapid screening methods of determining the status of cochlear function in individual mice. The resulting data will be made widely available on the internet in the form of databases and the relevant training manuals.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
7R01DC004476-03
Application #
6379552
Study Section
Special Emphasis Panel (ZMH1-BRB-I (01))
Program Officer
Watson, Bracie
Project Start
1999-09-27
Project End
2003-08-31
Budget Start
2001-09-29
Budget End
2003-08-31
Support Year
3
Fiscal Year
2001
Total Cost
$142,685
Indirect Cost
Name
University of Colorado Denver
Department
Otolaryngology
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045
Martin, Glen K; Vazquez, Ana E; Jimenez, Ana M et al. (2007) Comparison of distortion product otoacoustic emissions in 28 inbred strains of mice. Hear Res 234:59-72
Candreia, Claudia; Martin, Glen K; Stagner, Barden B et al. (2004) Distortion product otoacoustic emissions show exceptional resistance to noise exposure in MOLF/Ei mice. Hear Res 194:109-17
Howard, MacKenzie A; Stagner, Barden B; Lonsbury-Martin, Brenda L et al. (2002) Effects of reversible noise exposure on the suppression tuning of rabbit distortion-product otoacoustic emissions. J Acoust Soc Am 111:285-96