We plan to conduct immunocytochemical studies to localize fibronectin (FN) and collagen types I and III (Col I, Col III) in normal beagle periodontium and to determine changes in their distribution during ligature-induced and naturally-occurring periodontitis. We will measure FN, Col I, and Col III in gingival sulcular fluid and correlate these levels with inflammation as determined by light and electron microscopy and immunocytochemistry. Using the same methods, we will study the effect of inhibiting collagenase with minocycline on the level of FN, Col I, and Col III in gingival sulcular fluid. We expect to show that monitoring FN, Col I, and Col III in gingival crevicular fluid can be an accurate way to measure ongoing tissue destruction. If the beagle studies prove encouraging, we will begin similar investigations in humans. In a related project, we will use immunocytochemical methods to investigate the presence of FN in gingival plaque and calculus. We will continue autoradiographic studies of the penetration and incorporation into gingival epithelial cells of small metabolites applied to the gingival sulcular area. We hope to determine whether cell-to-cell communication and intracytoplasmic transport is a significant pathway in gingival epithelium. Preliminary findings suggest that further studies appear worthwhile. During the tenure of this grant, we have conducted numerous and basic studies of collagen secretion and fibroblast structure. We propose to continue these important studies by localizing fibroblasts rich in stress fibers by fluorescent microscopy with anti-actin and intermediate filaments with anti-vimentin antibodies. We will also examine the distribution of FN in relation to the development of fibronexus contacts by electron microscopy immunocytochemistry in areas previously identified by light microscopy to be sites rich in stress fiber development. We hope to correlate their presence with resistance to stress in the periodontal connective tissues. In vitro studies are also planned to further evaluate the polarity and locomotion of periodontal ligament fibroblasts in relation to the presence and distribution of stress fibers and fibronectin.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE003745-15
Application #
3218925
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1979-06-01
Project End
1990-05-31
Budget Start
1986-06-01
Budget End
1987-05-31
Support Year
15
Fiscal Year
1986
Total Cost
Indirect Cost
Name
State University New York Stony Brook
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794
Cho, M I; Garant, P R (1988) Ultrastructural evidence of directed cell migration during initial cementoblast differentiation in root formation. J Periodontal Res 23:268-76
Cho, M I; Garant, P R; Lee, Y L (1988) Immunocytochemical in vivo localization of fibronectin-rich contact sites on fibroblasts of normal periodontal ligament and inflamed gingiva. J Periodontal Res 23:230-8
Cho, M I; Garant, P R; Lee, Y L (1988) Periodontal ligament fibroblasts, preosteoblasts, and prechondrocytes express receptors for epidermal growth factor in vivo: a comparative radioautographic study. J Periodontal Res 23:287-94
Cho, M I; Lee, Y L; Garant, P R (1988) Radioautographic demonstration of receptors for epidermal growth factor in various cells of the oral cavity. Anat Rec 222:191-200
Cho, M I; Lee, Y L; Garant, P R (1987) Immunocytochemical localization of extracellular matrix components in beagle periodontium: I. Collagen types I and III in healthy gingival connective tissue. J Periodontal Res 22:313-9
Cho, M I; Garant, P R (1987) 3H-mannose utilization by fibroblasts of the periodontal ligament. Anat Rec 218:5-13
Cho, M I; Lee, Y L; Garant, P R (1985) Localization of fibronectin in gingival connective tissue of the beagle dog. Immunofluorescent light microscopic findings. J Periodontol 56:677-85
Cho, M I; Garant, P R (1985) Ultrastructural and cytochemical studies of the fate of unsecreted collagen precursors after administration of colchicine to mice. Acta Anat (Basel) 121:205-15
Cho, M I; Garant, P R (1985) Radioautographic analysis of [3H]-fucose utilization by mouse odontoblasts with emphasis on intracytoplasmic and plasma membrane glycoproteins. Arch Oral Biol 30:111-20
Cho, M I; Garant, P R (1985) Effects of L-azetidine-2-carboxylic acid on matrix secretion and Golgi structure in fibroblasts and osteoblasts of the mouse. Anat Rec 212:232-8