Fibroblasts are the predominant cells and collagen is the most abundant extracellular component in the periodontal ligament (PDL). The relationship between fibroblast polarity and orientation and the genesis of the 3-D architecture of the extracellular matrix is of increasing interest to cell biologists. Furthermore, the interrelationship between cytostructure and function during collagen secretion and fibrillogenesis contains information of significance not only to cell biology but also to a better understanding of the biology of the PDL. Greater understanding of PDL fibroblasts is of general importance to research in periodontal health. We propose to conduct research into six areas of fibroblast activity: 1) Studies of the cytological and synthetic aspects of fibroblast differentiation in relation to the development of the PDL fiber bundles. 2) Ultrastructural immunolocalization of C- and N- propeptides of collagen in fibroblasts and fibrils so as to learn more about their role in fibrillogenesis. 3) Immunocytochemical studies of collagen types I, III, V and fibronectin during fibroblast differentiation and PDL development. 4) Studies of the relationship between structure and function of the Golgiapparatus of PDL fibroblasts. 5) The localization of receptors for insulin, growth hormone and fibroblast growth factor on PDL fibroblast membranes during cytodifferentiation and PDL development. 6) Immunocytochemical localization of cathepsin D in periodontal ligament fibroblasts at the ultrastructural level.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE006165-05
Application #
3219901
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1983-02-01
Project End
1989-08-31
Budget Start
1987-09-01
Budget End
1988-08-31
Support Year
5
Fiscal Year
1987
Total Cost
Indirect Cost
Name
State University New York Stony Brook
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794
Cho, M I; Garant, P R (1988) Ultrastructural evidence of directed cell migration during initial cementoblast differentiation in root formation. J Periodontal Res 23:268-76
Cho, M I; Garant, P R; Lee, Y L (1988) Immunocytochemical in vivo localization of fibronectin-rich contact sites on fibroblasts of normal periodontal ligament and inflamed gingiva. J Periodontal Res 23:230-8
Cho, M I; Garant, P R; Lee, Y L (1988) Periodontal ligament fibroblasts, preosteoblasts, and prechondrocytes express receptors for epidermal growth factor in vivo: a comparative radioautographic study. J Periodontal Res 23:287-94
Cho, M I; Lee, Y L; Garant, P R (1988) Radioautographic demonstration of receptors for epidermal growth factor in various cells of the oral cavity. Anat Rec 222:191-200
Cho, M I; Garant, P R (1987) 3H-mannose utilization by fibroblasts of the periodontal ligament. Anat Rec 218:5-13
Cho, M I; Lee, Y L; Garant, P R (1987) Immunocytochemical localization of extracellular matrix components in beagle periodontium: I. Collagen types I and III in healthy gingival connective tissue. J Periodontal Res 22:313-9
Cho, M I; Garant, P R (1986) Radioautographic analysis of 3H-fucose utilization by fibroblasts of the periodontal ligament. J Periodontal Res 21:64-72
Cho, M I; Lee, Y L; Garant, P R (1985) Localization of fibronectin in gingival connective tissue of the beagle dog. Immunofluorescent light microscopic findings. J Periodontol 56:677-85
Cho, M I; Garant, P R (1985) Ultrastructural and cytochemical studies of the fate of unsecreted collagen precursors after administration of colchicine to mice. Acta Anat (Basel) 121:205-15
Cho, M I; Garant, P R (1985) Radioautographic analysis of [3H]-fucose utilization by mouse odontoblasts with emphasis on intracytoplasmic and plasma membrane glycoproteins. Arch Oral Biol 30:111-20

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