The long-range goal of the research is to ascertain the way in which early interactions between epithelial and mesenchymal tissues determine the different cellular phenotypes in the rat salivary glands. One line of research will use biochemical, immunochemical and imunocytochemical methods to further define and characterize secretory proteins that have a unique distribution in each different cell type of the major salivary glands and perhaps related glands, and use these as sensitive indicators of cellular phenotype. These studies will test the hypothesis that at least one secretory protein (SMG-A) is a characteristic gene product of both the neonatal submanibular and parotid glands, and later disappears from the submandibular and is maintained in the parotid as a major adult secretory product. They also will determine whether the neonatal Type III cells of the submandibular gland transform directly into adult mucous cells. Secondly, the unique but related pattern of proteins in each salivary cell type will be used to define the type of cellular differentiation that will occur in organ cultures and in novel epithelial and mesenchymal recombinations of salivary rudiments. Lastly, we shall determine whether intercalated ducts of the adult submandibular gland retain the potential to recapitulate the embryonic developmental program. We anticipate that the results will permit the formulation of useful hypotheses concerning both the organization and regulation of the genes for the salivary proteins, and the biochemical basis for the mesencymal specification of cellular phenotypes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
2R01DE006635-04
Application #
3220146
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1983-08-01
Project End
1991-11-30
Budget Start
1987-12-01
Budget End
1988-11-30
Support Year
4
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Howard University
Department
Type
Schools of Medicine
DUNS #
056282296
City
Washington
State
DC
Country
United States
Zip Code
20059
Ball, W D; Mirels, L; Hand, A R (2003) Psp and Smgb: a model for developmental and functional regulation in the rat major salivary glands. Biochem Soc Trans 31:777-80
Man, Y G; Ball, W D; Marchetti, L et al. (2001) Contributions of intercalated duct cells to the normal parenchyma of submandibular glands of adult rats. Anat Rec 263:202-14
Mirels, L; Miranda, A J; Ball, W D (1998) Characterization of the rat salivary-gland B1-immunoreactive proteins. Biochem J 330 ( Pt 1):437-44
Denny, P C; Ball, W D; Redman, R S (1997) Salivary glands: a paradigm for diversity of gland development. Crit Rev Oral Biol Med 8:51-75
Hand, A R; Sivakumar, S; Barta, I et al. (1996) Immunocytochemical studies of cell differentiation during rat salivary gland development. Eur J Morphol 34:149-54
Man, Y G; Ball, W D; Culp, D J et al. (1995) Persistence of a perinatal cellular phenotype in submandibular glands of adult rat. J Histochem Cytochem 43:1203-15
Ball, W D; Hand, A R; Moreira, J E et al. (1993) The B1-immunoreactive proteins of the perinatal submandibular gland: similarity to the major parotid gland protein, RPSP. Crit Rev Oral Biol Med 4:517-24
Mirels, L; Ball, W D (1992) Neonatal rat submandibular gland protein SMG-A and parotid secretory protein are alternatively regulated members of a salivary protein multigene family. J Biol Chem 267:2679-87
Ball, W D; Hand, A R; Moreira, J E (1991) A neonatal secretory protein associated with secretion granule membranes in developing rat salivary glands. J Histochem Cytochem 39:1693-706
Moreira, J E; Ball, W D; Mirels, L et al. (1991) Accumulation and localization of two adult acinar cell secretory proteins during development of the rat submandibular gland. Am J Anat 191:167-84

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