The purpose of this investigation is to study the mechanisms which regulate the biosynthesis and cellular processing of mucin-glycoproteins. In particular, we are interested in examining how these processes are influenced by normal physiological development. The transcription of the mRNA which directs the synthesis of these mucins will be studied by analyzing the biosynthesis of these specific messengers and quantitating their levels by hybridization to the appropriate cDNA probes. The course of mucin mRNA translation will be assessed by immunochemical quantitation of the newly synthesized peptide (apo-mucin) using antiserum of known specificity to the peptide core. Lastly, post-translational modification of the apo-mucins will be studied. First we will determine if apo-mucin is synthesized with a signal sequence is present (i.e.: pre-apo-mucin). In addition, we will attempt to identify the structural and conformational determinants which influence glycosylation of the apo-mucin. Finally, we will quantitate the levels of specific oligosaccharides and map their position on the peptide backbone, to determine if these parameters vary with normal, physiological development.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE006970-02
Application #
3220478
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1984-07-01
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost
Name
State University of New York at Buffalo
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260
Mirels, L; Bedi, G S; Dickinson, D P et al. (1987) Molecular characterization of glutamic acid/glutamine-rich secretory proteins from rat submandibular glands. J Biol Chem 262:7289-97
Pashley, D H; Kalathoor, S; Burnham, D (1986) The effects of calcium hydroxide on dentin permeability. J Dent Res 65:417-20
Tabak, L A; Mirels, L; Monte, L D et al. (1985) Isolation and characterization of a mucin-glycoprotein from rat submandibular glands. Arch Biochem Biophys 242:383-92