Abstract

The objectives of the proposed research are to determine the ultrastructural relationships and quantities of elements during the process of mineralization in cartilage and in long bones of embryonic chicks and rats. Cryo-techniques which minimize loss and dislocation of diffusible substances and quantitative electron probe analysis will be employed. Particular attention will be given to matrix vesicles. Elemental shifts in the calcium-transporting chorioallantoic membrane will also be analyzed. The role of carbonic anhydrase in the skeleton and in the chorioallantois will be studied. Vital stains will be developed to enhance the contrast of cryosections.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
9R01DE007431-08A1
Application #
3221068
Study Section
General Medicine B Study Section (GMB)
Project Start
1977-09-01
Project End
1988-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Pennsylvania State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
University Park
State
PA
Country
United States
Zip Code
16802