The basis of the proposal is a detailed examination of host- parasite interactions related to the mechanism of disease is patients infected with A. actinomycetemcomitans (Aa). The significance of the proposal is that humoral aspects of the immune response to be studied may provide an important host interface between the microorganism and manifestations of disease. To fully identify the specificity of the host responses to possible virulence factors of Aa, it will first be necessary to develop the methodology to characterize cell surface antigen preparations from the three serotypes of Aa, as well as methods for detailed analysis of human antibody responses to the individual antigens. Patients to be included in the studies will be those with evidence of periodontal disease and elevated systemic antibody responses to Aa. Studies of the antigenic specificities of the patients' antibody responses will be accomplished utilizing Western immunoblotting techniques. Initial cross-sectional studies of host responses will include IgG, IgM, IgA and IgE isotype antibodies, and the subclasses of IgG (IgGl, IgG2, IgG3, IgG4). The host response will be classified according to qualitative aspects of antigen specificities in each individual, as well as quantitative. definition of the level of response to each antigen in the preparations. Since it has been suggested that periodontal disease progresses by exacerbations and remissions, longitudinal measurements of host responses prior to, during and after the acute episodes will be examined. Recognizing that periodontal disease appears to be predominantly a disease localized to particular sites at any one time, further aims of the proposal will delineate the characteristics of the local antibody response. These analyses will include both cross-sectional studies of sites within a patient, as well as between patients and examination of longitudinal changes in antibody to Aa antigens at disease active and inactive sites, pre- and post-treatment. The responses in each study will then be compared to clinical parameters of disease and the antigenic burden of A. actinomycetemcomitans in the subgingival plaque in each patient. The results from these studies should provide a base of knowledge of the local and systemic antibody response to this proposed pathogen. Comparison of these responses to the disease parameters and to infection with A. actinomycetemcomitans should aid in understanding the mechanism of host interference with the pathogenicity of this microorganism.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE007809-04
Application #
3221604
Study Section
Oral Biology and Medicine Subcommittee 1 (OBM)
Project Start
1987-05-01
Project End
1991-04-30
Budget Start
1990-05-01
Budget End
1991-04-30
Support Year
4
Fiscal Year
1990
Total Cost
Indirect Cost
Name
University of Texas Health Science Center San Antonio
Department
Type
Schools of Dentistry
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229
Ebersole, J L; Cappelli, D; Steffen, M J (2000) Antigenic specificity of gingival crevicular fluid antibody to Actinobacillus actinomycetemcomitans. J Dent Res 79:1362-70
Celenligil-Nazliel, H; Kansu, E; Ebersole, J L (1999) Periodontal findings and systemic antibody responses to oral microorganisms in Behcet's disease. J Periodontol 70:1449-56
Ebersole, J L; Steffen, M J; Cappelli, D (1999) Longitudinal human serum antibody responses to outer membrane antigens of Actinobacillus actinomycetemcomitans. J Clin Periodontol 26:732-41
Dongari-Bagtzoglou, A I; Ebersole, J L (1998) Increased presence of interleukin-6 (IL-6) and IL-8 secreting fibroblast subpopulations in adult periodontitis. J Periodontol 69:899-910
Celenligil, H; Ebersole, J L (1998) Analysis of serum antibody responses to periodontopathogens in early-onset periodontitis patients from different geographical locations. J Clin Periodontol 25:994-1002
Celenligil, H; Eratalay, K; Kansu, E et al. (1998) Periodontal status and serum antibody responses to oral microorganisms in Sjogren's syndrome. J Periodontol 69:571-7
Dongari-Bagtzoglou, A I; Warren, W D; Berton, M T et al. (1997) CD40 expression by gingival fibroblasts: correlation of phenotype with function. Int Immunol 9:1233-41
Celenligil, H; Ebersole, J L (1997) Characteristics and responses of EBV immortalized B cells from periodontal disease patients. Oral Dis 3:262-71
Ebersole, J L; Cappelli, D; Steffen, M J et al. (1996) Host response assessment in recurring periodontitis. J Clin Periodontol 23:258-62
Hill, S J; Ebersole, J L (1996) The effect of lipopolysaccharide on growth factor-induced mitogenesis in human gingival fibroblasts. J Periodontol 67:1274-80

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