Abstract

The present proposal continues the ongoing efforts of this investigator to elucidate the biological effects of beta 1,4-galactosyltransferase (Gal Tase), and the expression of GTA-K on acinar cell growth response. The following specific aims have been proposed: (1) Elucidate the chromosomal structure of the GTA-K and determine the role of the regulatory sequences in the expression of this protein. (2) To study the biological effects of the GTA-K through site-directed mutagenesis of various functional domains. It is anticipated that these studies will provide new information about one possible mechanism involved in the regulation of acinar cell growth. A model which has been widely used, for almost three decades, to study acinar cell growth is the acinar cell proliferation in response to beta adrenergic stimulation. This study is aimed towards further characterizing the mechanisms associated with this proliferative event.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE008778-10
Application #
2713268
Study Section
Oral Biology and Medicine Subcommittee 1 (OBM)
Project Start
1997-08-01
Project End
2000-05-31
Budget Start
1998-06-01
Budget End
1999-05-31
Support Year
10
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Florida
Department
Dentistry
Type
Schools of Dentistry
DUNS #
073130411
City
Gainesville
State
FL
Country
United States
Zip Code
32611

  Publications

Cha, S; Peck, A B; Humphreys-Beher, M G (2002) Progress in understanding autoimmune exocrinopathy using the non-obese diabetic mouse: an update. Crit Rev Oral Biol Med 13:5-16
Koller, M M; Cowman, R A; Humphreys-Beher, M G et al. (2001) An analysis of parotid salivary gland function with desipramine and age in female NIA Fischer 344 rats. Exp Gerontol 36:141-57
Cha, S; van Blockland, S C; Versnel, M A et al. (2001) Abnormal organogenesis in salivary gland development may initiate adult onset of autoimmune exocrinopathy. Exp Clin Immunogenet 18:143-60
Koller, M M; Cowman, R A; Humphreys-Beher, M G et al. (2000) An analysis of submandibular salivary gland function with desipramine and age in female NIA Fischer 344 rats. Mech Ageing Dev 119:131-47
Dumbrigue, H B; Sandow, P L; Nguyen, K H et al. (2000) Salivary epidermal growth factor levels decrease in patients receiving radiation therapy to the head and neck. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 89:710-6
Nguyen, K H; Brayer, J; Cha, S et al. (2000) Evidence for antimuscarinic acetylcholine receptor antibody-mediated secretory dysfunction in nod mice. Arthritis Rheum 43:2297-306
Koller, M M; Maeda, N; Scarpace, P J et al. (2000) Desipramine changes salivary gland function, oral microbiota, and oral health in rats. Eur J Pharmacol 408:91-8
Koller, M M; Purushotham, K R; Maeda, N et al. (2000) Desipramine induced changes in salivary proteins, cultivable oral microbiota and gingival health in aging female NIA Fischer 344 rats. Life Sci 68:445-55
Oxford, G E; Jonsson, R; Olofsson, J et al. (1999) Elevated levels of human salivary epidermal growth factor after oral and juxtaoral surgery. J Oral Maxillofac Surg 57:154-8;discussion 158-9
Oxford, G E; Nguyen, K H; Alford, C E et al. (1998) Elevated salivary EGF levels stimulated by periodontal surgery. J Periodontol 69:479-84

Showing the most recent 10 out of 52 publications