Preliminary results have demonstrated both functional and morphological abnormalities in parotid glands of streptozotocin-diabetic rats compared with nondiabetic controls. Of particular, interest, the rate of secretion from the parotid gland in diabetic animals evoked by parasympathetic nerve stimulation were reduced, and numerous terminal axons evidenced signs of an initial axonopathy. The integrity of salivary gland function is dependent of an intact autonomic innervation, and these studies may provide valuable insights into the etiology, pathophysiology and treatment of diabetic autonomic neuropathy. However, differences observed between diabetic and control rats during parasympathetic stimulation may also result from altered cells responses to stimulation, or from other systemic effects of diabetes. Therefore, we propose to investigate the effects of diabetes on 1) the response of parotid glands in vivo and in vitro to adrenergic, cholinergic and peptidergic agonists, 2) the number, affinity and functional coupling of adrenergic, cholinergic and peptidergic receptors, and 3) the morphology and neurobiochemistry of autonomic nerves supplying the parotid glands. The goal of this and future research is to elucidate the pathogenesis of parotid gland dysfunction in diabetes, thereby determining the relative role of neural dysfunction and altered end-organ metabolism in this process.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
1R01DE009065-01
Application #
3222853
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1988-12-01
Project End
1991-11-30
Budget Start
1988-12-01
Budget End
1989-11-30
Support Year
1
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
Carpenter, G H; Proctor, G B; Anderson, L C et al. (2000) Immunoglobulin A secretion into saliva during dual sympathetic and parasympathetic nerve stimulation of rat submandibular glands. Exp Physiol 85:281-6
Garrett, J R; Zhang, X S; Proctor, G B et al. (1996) Apical secretion of rat submandibular tissue kallikrein continues in the absence of external stimulation: evidence for a constitutive secretory pathway. Acta Physiol Scand 157:299-304
Garrett, J R; Zhang, X S; Proctor, G B et al. (1996) Apical secretion of rat submandibular tissue kallikrein continues in the absence of external stimulation: evidence for a constitutive secretory pathway. Acta Physiol Scand 156:109-14
Anderson, L C; Garrett, J R; Zhang, X et al. (1995) Differential secretion of proteins by rat submandibular acini and granular ducts on graded autonomic nerve stimulations. J Physiol 485 ( Pt 2):503-11
Anderson, L C; Garrett, J R (1994) The effects of streptozotocin-induced diabetes on norepinephrine and cholinergic enzyme activities in rat parotid and submandibular glands. Arch Oral Biol 39:91-7
Anderson, L C; Suleiman, A H; Garrett, J R (1994) Morphological effects of diabetes on the granular ducts and acini of the rat submandibular gland. Microsc Res Tech 27:61-70
Anderson, L C; Garrett, J R; Suleiman, A H et al. (1993) In vivo secretory responses of submandibular glands in streptozotocin-diabetic rats to sympathetic and parasympathetic nerve stimulation. Cell Tissue Res 274:559-66
Anderson, L C; Garrett, J R; Suleiman, A H et al. (1992) Secretory oedema in diabetic submandibular glands during parasympathetic nerve stimulation: relationship to microvascular abnormalities in streptozotocin-treated rats. Comp Biochem Physiol Comp Physiol 103:145-9
Anderson, L C; Bevan, C A (1992) Effects of streptozotocin diabetes on amylase release and cAMP accumulation in rat parotid acinar cells. Arch Oral Biol 37:331-6
Garrett, J R; Anderson, L C (1991) Rat sublingual salivary glands: secretory changes on parasympathetic or sympathetic nerve stimulation and a reappraisal of the adrenergic innervation of striated ducts. Arch Oral Biol 36:675-83

Showing the most recent 10 out of 13 publications