The overall objective of this project is to determine the physiologic role of somatomedin-C (Sm-C) and other circulating forms of somatomedin in the regulation of growth. Specifically, we will clone and sequence the gene for the somatomedin-C precursor in order to study the mechanisms involved in the biosynthesis of somatomedin-C and the subsequent processing of the gene product. These studies will be carried out in human tumor cell lines which we have identified as producers of somatomedin-C-like activity. We will use the monoclonal antibodies which we have obtained against somatomedin-C production or storage. We will passively immunize young animals with these antibodies to determine the role of somatomedin-C in growth. The contributions of somatomedin-C to the mitogenic activity of serum will also be identified by immunoneutralization. We will continue to fractionate large quantities of human plasma to produce somatomedin-C, IFG-II, and related substances. We will determine the role of somatomedin-C in the feedback mechanisms controlling the secretion of growth hormone and other pituitary hormones and the mechanisms by which somatomedin-C interacts with other peptide growth factors in regulating cell replication.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK001022-31
Application #
3224255
Study Section
Endocrinology Study Section (END)
Project Start
1976-09-01
Project End
1987-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
31
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Type
Schools of Medicine
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599