One main objective of our research is to determine the role of caltrin in the development of the sperm acrosome reaction and in the acquisition of fertilizing capacity. We wish to learn if caltrin is found in all mammalian seminal fluid and we intend to document the different mechanisms used by different species to convert the inhibitory form to a conformation that enhances calcium uptake by the spermatozoa. A second main objective is to discover the mechanism by which thermogenic hormones function. The enzymes that are induced by thyroid hormone and dehydroepiandrosterone can be assigned roles that result in decreased metabolic efficiency and increased heat production. Our task is to establish a pathway by which reducing equivalents generated by malic enzyme can be used to convert dihydroxyacetone phosphate to sn-glycerol 3-phosphate. A third, but lesser, objective is to study the role of exercise training in the adaptation of enzyme activity to cope with the increased production of toxic oxygen radicals.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK010334-28
Application #
3224689
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1978-01-01
Project End
1994-06-30
Budget Start
1993-01-01
Budget End
1994-06-30
Support Year
28
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
Other Domestic Higher Education
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715