Abstract

The major thrust of the studies will be to identify both at cellular level and at the more macroscopic level of the surface mucous coat, the mechanism of protection of the surface cells of the stomach and duodenum against a hostile luminal environment. We will investigate those factors which influence the rate of alkaline secretion in isolated inhibited gastric fundus and in duodenum. The pH gradient across the surface mucous of gastric and duodenal mucosa and the thickness of the mucous gel will be measured with pH microelectrode or a slit lamp and pachymeter in vivo under a variety of experimental circumstances, especially those which affect secretion of mucous and HCO3-. In order to assess more carefully the intracellular events associated with alkaline secretion, and especially to elucidate the intricacies of gastric and duodenal ion transport, electron probe x-ray microanalysis will be used to measure intracellular elements in the various epithelial cells of these tissues. Attempts are under way to grow gastric surface and/or oxyntic cells in refined media with the intent that these will be layered on collagen or fibronectin to be studied in an Ussing chamber. This will allow a more accurate assessment of the detailed physiology of the complex epithelium of the stomach.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK015681-15
Application #
3225424
Study Section
Surgery and Bioengineering Study Section (SB)
Project Start
1977-01-01
Project End
1986-12-31
Budget Start
1986-01-01
Budget End
1986-12-31
Support Year
15
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02215

  Publications

Seo, Ji Hye; Fox, James G; Peek Jr, Richard M et al. (2011) N-methyl D-aspartate channels link ammonia and epithelial cell death mechanisms in Helicobacter pylori Infection. Gastroenterology 141:2064-75
Kang, Hye Won; Ribich, Scott; Kim, Brian W et al. (2009) Mice lacking Pctp /StarD2 exhibit increased adaptive thermogenesis and enlarged mitochondria in brown adipose tissue. J Lipid Res 50:2212-21
Hagen, Susan J; Ohtani, Masa; Zhou, Jin-Rong et al. (2009) Inflammation and foveolar hyperplasia are reduced by supplemental dietary glutamine during Helicobacter pylori infection in mice. J Nutr 139:912-8
Tashima, Kimihito; Zhang, Songhua; Ragasa, Regina et al. (2009) Hepatocyte growth factor regulates the development of highly pure cultured chief cells from rat stomach by stimulating chief cell proliferation in vitro. Am J Physiol Gastrointest Liver Physiol 296:G319-29
Hagen, Susan J; Yang, David X; Tashima, Kimihito et al. (2008) Epithelial cell expression of BCL-2 family proteins predicts mechanisms that regulate Helicobacter pylori-induced pathology in the mouse stomach. Lab Invest 88:1227-44
Ragasa, Regina; Nakamura, Eiji; Marrone, Lisa et al. (2007) Isothiocyanate inhibits restitution and wound repair after injury in the stomach: ex vivo and in vitro studies. J Pharmacol Exp Ther 323:1-9
Hagen, Susan J; Morrison, Sarah W; Law, Christina S et al. (2004) Restitution of the bullfrog gastric mucosa is dependent on a DIDS-inhibitable pathway not related to HCO3- ion transport. Am J Physiol Gastrointest Liver Physiol 286:G596-605
Ayabe, Tokiyoshi; Satchell, Donald P; Pesendorfer, Patrizia et al. (2002) Activation of Paneth cell alpha-defensins in mouse small intestine. J Biol Chem 277:5219-28
Abdul-Ghaffar Al-Shaibani, Tarik A; Hagen, Susan J (2002) Regulation of acid secretion and paracellular permeability by F-actin in the bullfrog, Rana catesbeiana. Am J Physiol Gastrointest Liver Physiol 282:G519-26
Nakamura, Eiji; Hagen, Susan J (2002) Role of glutamine and arginase in protection against ammonia-induced cell death in gastric epithelial cells. Am J Physiol Gastrointest Liver Physiol 283:G1264-75

Showing the most recent 10 out of 19 publications