The overall objective of this proposal is to clarify poorly understood aspects of the regulation of leucine catabolism by mammalian tissues. The work is focused on the regulation of the branched chain alpha-Ketoacid dehydrogenase (BCKDH) complex by its kinase and phosphatase. Proposed experiments are designed to answer four major questions: 1. What amin acid residues are involved in E1 binding of thiamin pyrophosphate (TPP), the essential cofactor for this component of the BCKDH complex? 2. What is the mechanism by which phosphorylation inhibits the dehydrogenase activity of BCKD E1? 3. How does BCKDH kinase recognize the site (E1-alpha-Ser293) that it phosphorylates on BCKDH E1? 4. What are the characteristics of the BCKDH phosphatase, and how is its activity toward the BCKDH complex regulated? The working hypotheses that will guide the work include: 1. A model based on the three-dimensional structure of transketolase identifies amino acid residues that may be involved in the binding of TPP by BCKDH E1; 2. Phosphorylation of E1-alpha-Ser293 by BCKDH kinase inactivates E1 by inhibiting TPP binding; and 3. The specificity of BCKDH kinase depends both on the amino acid residues spatially located in the vicinity of the E1 phosphorylation site and on the positioning of the kinase by E2 binding. The detailed specific aims are: 1. To establish amino acid residues critical to cofactor binding by the E1 component of the BCKDH complex; 2. To establish the molecular mechanism responsible for inhibition of BCKDH E1 activity by phosphorylation; 3. To establish the molecular basis for recognition of E1-alpha phosphorylation sites by BCKDH kinase; and 4. To clone, express, and characterize BCKDH phosphatase and determine its role in regulation of the activity state of the BCKDH complex.
| Shimomura, Yoshiharu; Honda, Takashi; Shiraki, Makoto et al. (2006) Branched-chain amino acid catabolism in exercise and liver disease. J Nutr 136:250S-3S |
| Joshi, Mandar A; Jeoung, Nam Ho; Obayashi, Mariko et al. (2006) Impaired growth and neurological abnormalities in branched-chain alpha-keto acid dehydrogenase kinase-deficient mice. Biochem J 400:153-62 |
| Harris, Robert A; Joshi, Mandar; Jeoung, Nam Ho et al. (2005) Overview of the molecular and biochemical basis of branched-chain amino acid catabolism. J Nutr 135:1527S-30S |
| Harris, Robert A; Joshi, Mandar; Jeoung, Nam Ho (2004) Mechanisms responsible for regulation of branched-chain amino acid catabolism. Biochem Biophys Res Commun 313:391-6 |
| Obayashi, Mariko; Shimomura, Yoshiharu; Nakai, Naoya et al. (2004) Estrogen controls branched-chain amino acid catabolism in female rats. J Nutr 134:2628-33 |
| Kobayashi, Rumi; Murakami, Taro; Obayashi, Mariko et al. (2002) Clofibric acid stimulates branched-chain amino acid catabolism by three mechanisms. Arch Biochem Biophys 407:231-40 |
| Harris, R A; Kobayashi, R; Murakami, T et al. (2001) Regulation of branched-chain alpha-keto acid dehydrogenase kinase expression in rat liver. J Nutr 131:841S-845S |
| Obayashi, M; Sato, Y; Harris, R A et al. (2001) Regulation of the activity of branched-chain 2-oxo acid dehydrogenase (BCODH) complex by binding BCODH kinase. FEBS Lett 491:50-4 |
| Baker, L J; Dorocke, J A; Harris, R A et al. (2001) The crystal structure of yeast thiamin pyrophosphokinase. Structure 9:539-46 |
| Timm, D E; Liu, J; Baker, L J et al. (2001) Crystal structure of thiamin pyrophosphokinase. J Mol Biol 310:195-204 |
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