The proper functioning of the urinary bladder is dependent on the delivery of a normal supply of blood, oxygen, and nutrients to the tissue. The complete or partial reduction of blood flow has been shown to seriously impair bladder function. Utilizing a variety of in-vivo and in-vitro techniques, we will investigate the relationship between the contractile function of the bladder and cellular metabolism, with special emphasis on intracellular energetics. There have been very few studies of the metabolic control of smooth muscle contraction except for studies of vascualr smooth muscle. It is questionable whether these studies of vascular smooth muscle can be directly related to bladder smooth muscle. Our proposed studies are designed to generate specific new information on: 1) carbohydrate metabolism, oxygen utilization, and high-energy phosphate metabolism of the bladder; 2) the relationship between the contractile and functional responses of the bladder to contractile agents and the metabolic effects of these agents; 3) the metabolic effects of direct electrical and neuronal stimulation; and, 4) the influence of age and sexual maturity on bladder function and metabolism. Our long-range goal is to identify specific metabolic defects that can either cause or result from specific bladder pathologies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK033559-02
Application #
3231988
Study Section
Surgery, Anesthesiology and Trauma Study Section (SAT)
Project Start
1984-12-01
Project End
1987-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Pennsylvania
Department
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Levin, Robert M; Hudson, Alan P (2004) The molecular genetic basis of mitochondrial malfunction in bladder tissue following outlet obstruction. J Urol 172:438-47
Nevel-McGarvey, C A; Rohrmann, D; Levin, R M et al. (1999) Mitochondrial and mitochondrial-related nuclear genetic function in rabbit urinary bladder following reversal of outlet obstruction. Mol Cell Biochem 197:161-72
Zhao, Y; Levin, S S; Wein, A J et al. (1997) Correlation of ischemia/reperfusion or partial outlet obstruction-induced spectrin proteolysis by calpain with contractile dysfunction in rabbit bladder. Urology 49:293-300
Yu, H I; Wein, A J; Levin, R M (1997) Contractile responses and calcium mobilization induced by muscarinic agonists in the rat urinary bladder: effects of age. Gen Pharmacol 28:623-8
Levin, R M; Hypolite, J A; Broderick, G A (1997) Evidence for a role of intracellular-calcium release in nitric oxide-stimulated relaxation of the rabbit corpus cavernosum. J Androl 18:246-9
Yu, H J; Wein, A J; Levin, R M (1996) Age-related differential susceptibility to calcium channel blocker and low calcium medium in rat detrusor muscle: response to field stimulation. Neurourol Urodyn 15:563-76
Chen, M W; Buttyan, R; Levin, R M (1996) Genetic and cellular response to unilateral ischemia of the rabbit urinary bladder. J Urol 155:732-7
Haugaard, N; Wein, A J; Chandy, B et al. (1996) Properties of Ca2(+)-Mg2+ ATP-ase in rabbit bladder muscle and mucosa: effect of urinary outlet obstruction. Neurourol Urodyn 15:555-61
Kwon, H Y; Longhurst, P A; Parsons, K et al. (1996) Effects of partial outlet obstruction on bladder-strip sensitivity to glucose deprivation: an in vitro study in the rat. World J Urol 14 Suppl 1:S38-42
Soyupak, B; Wein, A J; Levin, R M et al. (1996) Effect of ischemia of the rabbit bladder on Ca-Mg-activated ATP-ase activity. Neurourol Urodyn 15:666-71

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