Abstract

The first specific aim of this application is to determine how many insulin dependent diabetes mellitus patients can achieve insulin independence by islet transplants, and how long their grafts will last, in two groups of patients. The first study is to transplant 7 day, 24 degrees C cultured and cryopreserved islets into Type I patients with established kidney transplants with 7 days of MALG and basal immunosuppression. The second study is to transplant 7 day, 24 degrees C cultured and cryopreserved islets into patients getting their kidney transplant. With this information obtained, the next step will be to determine the ability of immunoalteration techniques to permit transplantation without long term immunosuppression. The second specific aim is to transplant 7 day cultured, cryopreserved islets into Type I patients entering renal failure who will have their islets treated with either culture and cryopreservation, multiple donors, UV light therapy, or specific anti-class II antibodies prior to transplantation. The successful completion of these studies will determine the practical feasibility of islet transplantation to achieve its long term objective.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
2R01DK040465-04
Application #
3240756
Study Section
Surgery, Anesthesiology and Trauma Study Section (SAT)
Project Start
1988-09-01
Project End
1994-08-31
Budget Start
1991-09-01
Budget End
1992-08-31
Support Year
4
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Urbanek, Margrit; Du, Yangzhu; Silander, Kaisa et al. (2003) Variation in resistin gene promoter not associated with polycystic ovary syndrome. Diabetes 52:214-7
Swanson, C J; Olack, B J; Flavin, K S et al. (1997) Comparison of transplanted islets in patients with functioning versus nonfunctioning allografts. Transplant Proc 29:2241-2
Marchetti, P; Scharp, D W; Giannarelli, R et al. (1996) Metformin potentiates glucose-stimulated insulin secretion. Diabetes Care 19:781-2
Brennan, D C; Scharp, D W; White, N H et al. (1995) Modification of methodology results in improvement in simultaneous kidney-islet success. Transplant Proc 27:1349-50
Scharp, D W; Swanson, C J; Olack, B J et al. (1994) Protection of encapsulated human islets implanted without immunosuppression in patients with type I or type II diabetes and in nondiabetic control subjects. Diabetes 43:1167-70
Marchetti, P; Scharp, D W; Mclear, M et al. (1994) Pulsatile insulin secretion from isolated human pancreatic islets. Diabetes 43:827-30
Brelje, T C; Scharp, D W; Lacy, P E et al. (1993) Effect of homologous placental lactogens, prolactins, and growth hormones on islet B-cell division and insulin secretion in rat, mouse, and human islets: implication for placental lactogen regulation of islet function during pregnancy. Endocrinology 132:879-87
Scharp, D W; Lacy, P E; McLear, M et al. (1992) The bioburden of 590 consecutive human pancreata for islet transplant research. Transplant Proc 24:974-5
Koranyi, L I; Tanizawa, Y; Welling, C M et al. (1992) Human islet glucokinase gene. Isolation and sequence analysis of full-length cDNA. Diabetes 41:807-11
Scharp, D W; Lacy, P E; Santiago, J V et al. (1991) Results of our first nine intraportal islet allografts in type 1, insulin-dependent diabetic patients. Transplantation 51:76-85

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