Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK043595-05
Application #
3244962
Study Section
Diabetes, Endocrinology and Metabolic Diseases B Subcommittee (DDK)
Project Start
1990-09-25
Project End
1994-09-29
Budget Start
1994-09-01
Budget End
1994-09-29
Support Year
5
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Whittier Institute for Diabetes & Endoc
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Grimshaw, C E; Lai, C J (1996) Oxidized aldose reductase: in vivo factor not in vitro artifact. Arch Biochem Biophys 327:89-97
Grimshaw, C E; Bohren, K M; Lai, C J et al. (1995) Human aldose reductase: subtle effects revealed by rapid kinetic studies of the C298A mutant enzyme. Biochemistry 34:14366-73
Grimshaw, C E; Bohren, K M; Lai, C J et al. (1995) Human aldose reductase: pK of tyrosine 48 reveals the preferred ionization state for catalysis and inhibition. Biochemistry 34:14374-84
Grimshaw, C E; Bohren, K M; Lai, C J et al. (1995) Human aldose reductase: rate constants for a mechanism including interconversion of ternary complexes by recombinant wild-type enzyme. Biochemistry 34:14356-65
Barski, O A; Gabbay, K H; Grimshaw, C E et al. (1995) Mechanism of human aldehyde reductase: characterization of the active site pocket. Biochemistry 34:11264-75
Grimshaw, C E; Lai, C J (1995) Stopped-flow studies of human aldose reductase reveal which enzyme form predominates during steady-state turnover in either reaction direction. Adv Exp Med Biol 372:229-40
Bohren, K M; Grimshaw, C E; Lai, C J et al. (1994) Tyrosine-48 is the proton donor and histidine-110 directs substrate stereochemical selectivity in the reduction reaction of human aldose reductase: enzyme kinetics and crystal structure of the Y48H mutant enzyme. Biochemistry 33:2021-32
Grimshaw, C E (1992) Aldose reductase: model for a new paradigm of enzymic perfection in detoxification catalysts. Biochemistry 31:10139-45
Bohren, K M; Grimshaw, C E; Gabbay, K H (1992) Catalytic effectiveness of human aldose reductase. Critical role of C-terminal domain. J Biol Chem 267:20965-70
Grimshaw, C E (1991) Enantiospecific change in products for aldose reductase-mediated reaction of glyceraldehyde with bound NADP+. Biochem Biophys Res Commun 175:943-8