The long-term objective of this proposal is to determine-the role of modifiers of malonyl-CoA and succinate synthesis and regulators of glutamate dehydrogenase activity in insulin release from pancreatic islets. In general, the research design will be to incubate islets with secretagogues and/or inhibitors of insulin release and measure insulin release plus the levels of key metabolites which can provide insight into the mechanism of insulin release. The methods are the same as we have used previously and involve assays of insulin release and measurement of key metabolites with high pressure liquid chromatography or other standard methods. The results of these studies will further enhance our understanding of insulin release and hopefully lead to better oral hypoglycemic agents for the treatment of diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
1R01DK047041-01A1
Application #
2146356
Study Section
Metabolism Study Section (MET)
Project Start
1994-04-01
Project End
1997-03-31
Budget Start
1994-04-01
Budget End
1995-03-31
Support Year
1
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Pharmacology
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Simons Jr, S Stoney (2008) What goes on behind closed doors: physiological versus pharmacological steroid hormone actions. Bioessays 30:744-56
Szapary, Daniele; Song, Liang-Nian; He, Yuangzheng et al. (2008) Differential modulation of glucocorticoid and progesterone receptor transactivation. Mol Cell Endocrinol 283:114-26
Prabhakar, P; Laboy, J I; Wang, J et al. (1998) Effect of NADH-X on cytosolic glycerol-3-phosphate dehydrogenase. Arch Biochem Biophys 360:195-205