Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK049655-02
Application #
2150511
Study Section
Biophysical Chemistry Study Section (BBCB)
Project Start
1995-06-01
Project End
1998-04-30
Budget Start
1996-05-01
Budget End
1997-04-30
Support Year
2
Fiscal Year
1996
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Biomedical Engineering
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Rinne, Johanna; Albarran, Brian; Jylhava, Juulia et al. (2007) Internalization of novel non-viral vector TAT-streptavidin into human cells. BMC Biotechnol 7:1
Le Trong, Isolde; Humbert, Nicolas; Ward, Thomas R et al. (2006) Crystallographic analysis of a full-length streptavidin with its C-terminal polypeptide bound in the biotin binding site. J Mol Biol 356:738-45
Albarran, Brian; To, Richard; Stayton, Patrick S (2005) A TAT-streptavidin fusion protein directs uptake of biotinylated cargo into mammalian cells. Protein Eng Des Sel 18:147-52
Hamblett, Kevin J; Press, Oliver W; Meyer, Damon L et al. (2005) Role of biotin-binding affinity in streptavidin-based pretargeted radioimmunotherapy of lymphoma. Bioconjug Chem 16:131-8
Le Trong, Isolde; Freitag, Stefanie; Klumb, Lisa A et al. (2003) Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin. Acta Crystallogr D Biol Crystallogr 59:1567-73
Le Trong, Isolde; McDevitt, Todd C; Nelson, Kjell E et al. (2003) Structural characterization and comparison of RGD cell-adhesion recognition sites engineered into streptavidin. Acta Crystallogr D Biol Crystallogr 59:828-34
Hamblett, Kevin J; Kegley, Brian B; Hamlin, Don K et al. (2002) A streptavidin-biotin binding system that minimizes blocking by endogenous biotin. Bioconjug Chem 13:588-98
Dixon, Richard W; Radmer, Randall J; Kuhn, Bernd et al. (2002) Theoretical and experimental studies of biotin analogues that bind almost as tightly to streptavidin as biotin. J Org Chem 67:1827-37
Stayton, P S; Hoffman, A S; Murthy, N et al. (2000) Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics. J Control Release 65:203-20
Hyre, D E; Le Trong, I; Freitag, S et al. (2000) Ser45 plays an important role in managing both the equilibrium and transition state energetics of the streptavidin-biotin system. Protein Sci 9:878-85

Showing the most recent 10 out of 20 publications