Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK049726-02
Application #
2150618
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1995-06-01
Project End
1999-05-31
Budget Start
1996-06-01
Budget End
1997-05-31
Support Year
2
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Colorado Denver
Department
Pediatrics
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045
Dwyer, T M; Zhang, L; Muller, M et al. (1999) The functions of the flavin contact residues, alphaArg249 and betaTyr16, in human electron transfer flavoprotein. Biochim Biophys Acta 1433:139-52
Dwyer, T M; Mortl, S; Kemter, K et al. (1999) The intraflavin hydrogen bond in human electron transfer flavoprotein modulates redox potentials and may participate in electron transfer. Biochemistry 38:9735-45
Roberts, D L; Salazar, D; Fulmer, J P et al. (1999) Crystal structure of Paracoccus denitrificans electron transfer flavoprotein: structural and electrostatic analysis of a conserved flavin binding domain. Biochemistry 38:1977-89
Griffin, K J; Degala, G D; Eisenreich, W et al. (1998) 31P-NMR spectroscopy of human and Paracoccus denitrificans electron transfer flavoproteins, and 13C- and 15N-NMR spectroscopy of human electron transfer flavoprotein in the oxidised and reduced states. Eur J Biochem 255:125-32
Salazar, D; Zhang, L; deGala, G D et al. (1997) Expression and characterization of two pathogenic mutations in human electron transfer flavoprotein. J Biol Chem 272:26425-33
Griffin, K J; Dwyer, T M; Manning, M C et al. (1997) alphaT244M mutation affects the redox, kinetic, and in vitro folding properties of Paracoccus denitrificans electron transfer flavoprotein. Biochemistry 36:4194-202
Roberts, D L; Frerman, F E; Kim, J J (1996) Three-dimensional structure of human electron transfer flavoprotein to 2.1-A resolution. Proc Natl Acad Sci U S A 93:14355-60
Roberts, D L; Herrick, K R; Frerman, F E et al. (1995) Crystallization and preliminary X-ray analysis of electron transfer flavoproteins from human and Paracoccus denitrificans. Protein Sci 4:1654-7