Abstract

The specific aims of the proposal are (1) a C. jejuni cadF mutant will be generated by homologous recombination; (2) C. jejuni binding deficient mutants will be isolated by testing M-methyl-N'-nitro-N-nitrosoguanidine and ultra violet-irradiated bacteria in binding assays; (3) C. jejuni insertional knockout mutants will be created in genes of interest using allelic exchange mutagenesis; (4) C. jejuni insertional mutants deficient in their ability to enter INT 407 epithelial cells will be tested for their in vivo pathogenic behavior using a newborn piglet model.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK050567-03
Application #
2905796
Study Section
Bacteriology and Mycology Subcommittee 2 (BM)
Program Officer
Hamilton, Frank A
Project Start
1997-06-01
Project End
2001-05-15
Budget Start
1999-05-16
Budget End
2001-05-15
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Washington State University
Department
Microbiology/Immun/Virology
Type
Schools of Arts and Sciences
DUNS #
041485301
City
Pullman
State
WA
Country
United States
Zip Code
99164

  Publications

Mamelli, Laurent; Pages, Jean-Marie; Konkel, Michael E et al. (2006) Expression and purification of native and truncated forms of CadF, an outer membrane protein of Campylobacter. Int J Biol Macromol 39:135-40
Raphael, Brian H; Pereira, Sonia; Flom, Gary A et al. (2005) The Campylobacter jejuni response regulator, CbrR, modulates sodium deoxycholate resistance and chicken colonization. J Bacteriol 187:3662-70
Konkel, Michael E; Klena, John D; Rivera-Amill, Vanessa et al. (2004) Secretion of virulence proteins from Campylobacter jejuni is dependent on a functional flagellar export apparatus. J Bacteriol 186:3296-303
Rivera-Amill, V; Kim, B J; Seshu, J et al. (2001) Secretion of the virulence-associated Campylobacter invasion antigens from Campylobacter jejuni requires a stimulatory signal. J Infect Dis 183:1607-16
Konkel, M E; Tilly, K (2000) Temperature-regulated expression of bacterial virulence genes. Microbes Infect 2:157-66
Konkel, M E; Mixter, P F (2000) Flow cytometric detection of host cell apoptosis induced by bacterial infection. Methods Cell Sci 22:209-15
Ziprin, R L; Young, C R; Stanker, L H et al. (1999) The absence of cecal colonization of chicks by a mutant of Campylobacter jejuni not expressing bacterial fibronectin-binding protein. Avian Dis 43:586-9
Rivera-Amill, V; Konkel, M E (1999) Secretion of Campylobacter jejuni Cia proteins is contact dependent. Adv Exp Med Biol 473:225-9
Gray, S A; Konkel, M E (1999) Codon usage in the A/T-rich bacterium Campylobacter jejuni. Adv Exp Med Biol 473:231-5
Konkel, M E; Gray, S A; Kim, B J et al. (1999) Identification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. J Clin Microbiol 37:510-7

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