The mechanisms controlling human placental GH and CS gene activation and repression in placenta will be investigated. In the proposed studies, the following aims will be pursued: 1) the individual enhansons characterizing the enhancer will be investigated; 2) multiple enhancer interactions will be characterized in placental and pituitary cells; 3) the relationship of the enhancer interactions with pituitary-specific P element repressors will be investigated; 4) the chromatin structure and matrix attachment regions in the CS/GH locus will be defined; and 5) enhancer (CSEF-1) and inhibitor (CSInrF) proteins will be isolated, sequenced, and cloned.
| Jiang, S W; Desai, D; Khan, S et al. (2000) Cooperative binding of TEF-1 to repeated GGAATG-related consensus elements with restricted spatial separation and orientation. DNA Cell Biol 19:507-14 |
| Jiang, S W; Trujillo, M A; Sakagashira, M et al. (2000) Novel human TEF-1 isoforms exhibit altered DNA binding and functional properties. Biochemistry 39:3505-13 |
| Jiang, S W; Wu, K; Eberhardt, N L (1999) Human placental TEF-5 transactivates the human chorionic somatomammotropin gene enhancer. Mol Endocrinol 13:879-89 |
| Jiang, S W; Eberhardt, N L (1997) The human chorionic somatomammotropin enhancers form a composite silencer in pituitary cells in vitro. Mol Endocrinol 11:1233-44 |
| Jiang, S W; Trujillo, M A; Eberhardt, N L (1997) Human chorionic somatomammotropin enhancer function is mediated by cooperative binding of TEF-1 and CSEF-1 to multiple, low-affinity binding sites. Mol Endocrinol 11:1223-32 |
