Currently, virtually nothing is known about the existence and role of T lymphocytes, mast cells and their products in non bacterial chronic prostatitis (NBCP). Thus, it is essential that a systematic characterization of these cells and their products in NBCP be undertaken to fill this major gap in our knowledge of this inflammatory disease. Thus, we propose to: 1) systematically characterize T lymphocyte subpopulations in NBCP and non NBCP patients (for clinical criteria, see Patient Population below); 2) determine the role of CD4 Th2-related cytokines, particularly IL3 in promoting mast cell growth and differentiation in NBCP, and 3) to determine the role of mast cells and their products in NBCP and non NBCP. We hypothesize that NBCP is an inflammatory disease of the prostate that results from an imbalance of specific T-lymphocyte subpopulations. To test this hypothesis, we have developed the following specific aims: (1) to isolate, characterize and correlate T cells and T cell clones from fluids (blood, urine, prostate fluids) and tissue from NBCP, non NBCP and normal patient; (2) to characterize and correlate the distribution of CD4 Th1 and CD4 Th2 lymphocytes and associated cytokines in fluids (blood, urine, prostate fluids) and tissue from NBCP, non NBCP and normal patients; and (3) to characterize and correlate the distribution of mast cells and mast cell-related factors in NBCP, non NBCP and normal patients.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
1R01DK052718-01
Application #
2018220
Study Section
Special Emphasis Panel (SRC (05))
Project Start
1997-07-07
Project End
2001-05-31
Budget Start
1997-07-07
Budget End
1998-05-31
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Connecticut
Department
Pathology
Type
Schools of Dentistry
DUNS #
City
Farmington
State
CT
Country
United States
Zip Code
06030
Miller, Lauri J; Fischer, Kateri A; Goralnick, Sandra J et al. (2002) Interleukin-10 levels in seminal plasma: implications for chronic prostatitis-chronic pelvic pain syndrome. J Urol 167:753-6
Miller, Lauri J; Fischer, Kateri A; Goralnick, Sandra J et al. (2002) Nerve growth factor and chronic prostatitis/chronic pelvic pain syndrome. Urology 59:603-8
Ferrer, F A; Miller, L J; Lindquist, R et al. (1999) Expression of vascular endothelial growth factor receptors in human prostate cancer. Urology 54:567-72