Biotin containing enzymes are found in all organisms and catalyze key steps in metabolic pathways. In humans, deficiencies in these enzymes can have serious, or catastrophic, consequences. In spite of continuing mechanistic and medical interest, little is known of the detailed structure and chemistry of this closely related family of proteins. The strategic goals of this research are to define the atomic details of the 1.3 S subunit of transcarboxylase (TC) and of its intermediate which has a carboxylate group transiently bound to the biotin cofactor. Multidimensional heteronuclear NMR will be used to solve the structures of the holo- and apo- 1.3 S protein in solution. The proteins will be labeled with 15N and/or 13C. The properties of the biotin co-factor on the protein, which relate to its function as a carboxylate acceptor, transporter and donor, will be elucidated by Raman and FTIR difference spectroscopies and by NMR. The structure and electron distribution in the carboxy-biotin complex on the 1.3 S subunit will be determined also by a combination of vibrational and NMR spectroscopies. In addition, changes in the protein s structure upon biotin carboxylation will be probed by NMR. Recent FTIR data indicate that after the CO2 moiety leaves the biotin ring on 1.3S via spontaneous decarboxylation, it remains associated witht he protein as an, as yet, uncharacterized complex. The complex will be identified using virbational spectroscopic techniques and NMR, in addition to experiments aimed at trapping the complex in a stable form using CH2N2. As a test of catalytic competence, the novel complex will be reacted with pyruvate in the presence of the 5S subunit to see if oxalacetate is formed. Vibrational spectroscopic analysis of biotin model compounds, of carboxy-biotin compounds and their isotopomers will be combined with quantum mechanical calculations to provide a basis for interpreting the data for biotin and carboxy-biotin on 1.3S.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK053053-04
Application #
6178005
Study Section
Physical Biochemistry Study Section (PB)
Program Officer
Laughlin, Maren R
Project Start
1997-08-15
Project End
2002-06-30
Budget Start
2000-07-01
Budget End
2002-06-30
Support Year
4
Fiscal Year
2000
Total Cost
$233,096
Indirect Cost
Name
Case Western Reserve University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
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