The long term goals of the project are to define the physiology and biochemistry of fat digestion.
The specific aims of the proposal are: (1) define the role of pancreatic lipase related protein 2 (PLRP2), a homologue of pancreatic triglyceride lipase, in fat digestion at various ages by characterizing fat utilization in PLRP2 deficient mice. The approach to this aim will be to determine fecal fat excretion in PLRP2 deficient mice at various stages of development and after being fed different levels of fat. (2) delineate the role of colipase in dietary fat digestion by ablating the gene encoding procolipase in mice. These mice will be created and the absence of colipase expression determined by mRNA and protein analysis. Fat absorption will be ascertained in the deficient mice; (3) demonstrate that enterostatin, the activation peptide of procolipase, mediates satiety and controls obesity by creating enterostatin deficient mice with gene ablation and transgenic techniques; and (4) characterize the receptor for enterostatin on neuronal and nonneuronal cells by measuring binding of radiolabeled enterostatin. Detailed knowledge of the receptor properties may reveal a novel approach to obesity treatment and fat satiety.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK053100-02
Application #
2906118
Study Section
Nutrition Study Section (NTN)
Program Officer
May, Michael K
Project Start
1998-06-01
Project End
2001-05-31
Budget Start
1999-06-01
Budget End
2000-05-31
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Washington University
Department
Pediatrics
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
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Berna, Marc J; Tapia, Jose A; Sancho, Veronica et al. (2007) Progress in developing cholecystokinin (CCK)/gastrin receptor ligands that have therapeutic potential. Curr Opin Pharmacol 7:583-92
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