Abstract

This Bioengineering Research Grant proposal addresses the development of analytical and computational methodologies to model and analyze light scattering signals, which can be measured non-invasively from living cells and tissues. Light scattering plays a fundamental role in light trainsport in turbid media such as biological tissues and can provide valuable diagnostic information about the structure and composition of a tissue. Therefore, understanding tissue light scattering will help advance many modalities of optical imaging and diagnostics. However, the mechanisms of light scattering in cells and tissues are still poorly understood. We propose to develop novel analytical and numerical methods to analyze light scattering signals and identify the origins of tissue light scattering utilizing our robust computational finite-difference time-domain (FDTD) and pseudo-spectral time-domain (PSTD) modeling and our novel analytical methods. These studies will be built upon our recent development of analytical and numerical methods to describe spectral and angular properties of light scattering by inhomogeneous and non-spherical particles. The theoretical and computational studies will be accompanied by comprehensive experimental measurements of light scattering in living cells using our recently developed light scattering instrument, which provides multi-dimensional data about the light scattering.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
1R01EB003682-01A2
Application #
6973570
Study Section
Biomedical Imaging Technology Study Section (BMIT)
Program Officer
Zhang, Yantian
Project Start
2005-07-12
Project End
2009-04-30
Budget Start
2005-07-12
Budget End
2006-04-30
Support Year
1
Fiscal Year
2005
Total Cost
$233,844
Indirect Cost

  Institution

Name
Northwestern University at Chicago
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
160079455
City
Evanston
State
IL
Country
United States
Zip Code
60201

  Publications

Khan, Sheema; Zafar, Nadeem; Khan, Shabia S et al. (2018) Clinical significance of MUC13 in pancreatic ductal adenocarcinoma. HPB (Oxford) 20:563-572
Khan, S; Sikander, M; Ebeling, M C et al. (2017) MUC13 interaction with receptor tyrosine kinase HER2 drives pancreatic ductal adenocarcinoma progression. Oncogene 36:491-500
Swain, Timothy D; DuBois, Emily; Goldberg, Scott J et al. (2017) Bleaching response of coral species in the context of assemblage response. Coral Reefs 36:395-400
Sahay, Peeyush; Shukla, Pradeep K; Ghimire, Hemendra M et al. (2017) Quantitative analysis of nanoscale intranuclear structural alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy imaging. Phys Biol 14:026001
Swain, Timothy D; DuBois, Emily; Gomes, Andrew et al. (2016) Skeletal light-scattering accelerates bleaching response in reef-building corals. BMC Ecol 16:10
Swain, Timothy D; Vega-Perkins, Jesse B; Oestreich, William K et al. (2016) Coral bleaching response index: a new tool to standardize and compare susceptibility to thermal bleaching. Glob Chang Biol 22:2475-88
Radosevich, Andrew J; Eshein, Adam; Nguyen, The-Quyen et al. (2015) Subdiffusion reflectance spectroscopy to measure tissue ultrastructure and microvasculature: model and inverse algorithm. J Biomed Opt 20:097002
Mutyal, Nikhil N; Radosevich, Andrew J; Bajaj, Shailesh et al. (2015) In vivo risk analysis of pancreatic cancer through optical characterization of duodenal mucosa. Pancreas 44:735-41
Patel, Mihir; Gomes, Andrew; Ruderman, Sarah et al. (2014) Polarization gating spectroscopy of normal-appearing duodenal mucosa to detect pancreatic cancer. Gastrointest Endosc 80:786-93.e1-2
Cherkezyan, Lusik; Stypula-Cyrus, Yolanda; Subramanian, Hariharan et al. (2014) Nanoscale changes in chromatin organization represent the initial steps of tumorigenesis: a transmission electron microscopy study. BMC Cancer 14:189

Showing the most recent 10 out of 62 publications