The goal of this project is to develop a general, modular fiber optic sensor system capable of quantifying biologically relevant levels of specific proteins in vitro. The sensor system is designed for rapid bedside analysis, for primary diagnoses in rural or technologically poor environments, and timely diagnoses by first responders in the field. The sensor system will be faster than existing commercially available fieldable sensors while maintaining the sensitivity and selectivity of common laboratory tests for the target proteins. By constructing the sensors on optical fibers, the sensor system can employ inexpensive, interchangeable sensing elements for specific tests with simple automated procedures for preparing the sensing elements for operation. The sensors use surface plasmon resonance (SPR) on optical fibers to detect the markers through immunoassays, and are encapsulated in a thin film polymer housing that prevents cellular fouling of the sensing region. Phage display will be employed to collect improved protein specific receptors with desired binding sensitivities for target applications. Because the assay is conducted on the surface of an optical fiber, the probes can be used as immersion probes in vitro. Four target example applications around which we will base development and optimization of the sensor system have been chosen: proteins for detecting and determining the severity of myocardial infarctions, proteins for assessing ability of wounds to heal, proteins associated with ischemic stroke; proteins for detecting spinal motor atrophy. Sensor optimization will address specific biological matrices where these proteins are found. These example applications are selected to demonstrate the flexibility and relevancy of the sensing system, but should not be construed as the limiting cases of its use. In fact, the antibody-based immunoassay approach has applications far beyond those considered here.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB004761-03
Application #
7076141
Study Section
Special Emphasis Panel (ZRG1-ISD (01))
Program Officer
Korte, Brenda
Project Start
2004-09-01
Project End
2007-02-01
Budget Start
2006-07-01
Budget End
2007-02-01
Support Year
3
Fiscal Year
2006
Total Cost
$243,045
Indirect Cost
Name
Arizona State University-Tempe Campus
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
943360412
City
Tempe
State
AZ
Country
United States
Zip Code
85287
Staton, Sarah J R; Jones, Paul V; Ku, Ginger et al. (2012) Manipulation and capture of Aýý amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP). Analyst 137:3227-9
Kenyon, Stacy M; Weiss, Noah G; Hayes, Mark A (2012) Using electrophoretic exclusion to manipulate small molecules and particles on a microdevice. Electrophoresis 33:1227-35
Zou, Qiongjing; Menegazzo, Nicola; Booksh, Karl S (2012) Development and investigation of a dual-pad in-channel referencing surface plasmon resonance sensor. Anal Chem 84:7891-8
Weiss, Noah G; Jones, Paul V; Mahanti, Prasun et al. (2011) Dielectrophoretic mobility determination in DC insulator-based dielectrophoresis. Electrophoresis 32:2292-7
Weiss, Noah G; Hayes, Mark A; Garcia, Antonio A et al. (2011) Isoelectric focusing in a drop. Langmuir 27:494-8
Weiss, Noah G; Jarvis, Jason W; Nelson, Randall W et al. (2011) Examining serum amyloid P component microheterogeneity using capillary isoelectric focusing and MALDI-MS. Proteomics 11:106-13
Mahanti, Prasun; Taylor, Thomas; Hayes, Mark A et al. (2011) Improved detectability and signal strength for rotating phase fluorescence immunoassays through image processing. Analyst 136:365-73
Menegazzo, Nicola; Kegel, Laurel L; Kim, Yoon-Chang et al. (2010) Characterization of a variable angle reflection Fourier transform infrared accessory modified for surface plasmon resonance spectroscopy. Appl Spectrosc 64:1181-6
Staton, Sarah J R; Chen, Kang Ping; Taylor, Thomas J et al. (2010) Characterization of particle capture in a sawtooth patterned insulating electrokinetic microfluidic device. Electrophoresis 31:3634-41
Farrell, Megan; Beaudoin, Stephen (2010) Surface forces and protein adsorption on dextran- and polyethylene glycol-modified polydimethylsiloxane. Colloids Surf B Biointerfaces 81:468-75

Showing the most recent 10 out of 14 publications