Exposure of therapeutic and diagnostic medical devices to biological fluids is often accompanied by interfacial adsorption of proteins, cells and microorganisms. Biofouling of surfaces can lead to compromised device performance, increased cost, and in some cases may be life-threatening to the patient. The elimination or minimization of nonspecific biomolecule-material interactions is therefore an integral part of refining the biological performance of current and future biomaterials. Although several antifouling polymer coatings have enjoyed short-term success in preventing protein and cell adsorption on surfaces, none have proven ideal for conferring long-term biofouling resistance. The primary goal of this study is to design and synthesize novel long-lasting antifouling polymers with chemical and structural characteristics optimal for preventing protein fouling at biointerfaces. These polymers consist of two distinct domains coupled together- an anchoring domain inspired by the adhesive proteins secreted by mussels for attachment to marine surfaces, and an antifouling poly(N-substituted glycine) """"""""peptoid"""""""" segment designed to resist protein and cellular attachment. Peptidomimetic polymers with a variety of compositions, lengths, and architectures will be synthesized, and high sensitivity protein adsorption experiments will be performed to test the protein resistance of these polymers. The protein adsorption experiments will be both guided by, and confirmed with, theoretical calculations of the systems using a molecular theory that is particularly well-suited to investigating protein interactions with grafted polymers. Such systematic coupled experimental/theoretical investigations are difficult to accomplish with traditional synthetic polymers, but are facilitated in our case by the precise control of peptidomimetic polymer architecture, molecular weight, and composition. Outcomes of this study will include new insights into fundamental properties of antifouling polymers, as well as identification of new biologically inspired polymers capable of limiting protein and cell fouling of therapeutic and diagnostic device surfaces. PUBLIC HEALTH REVELANCE In this study we will combine theoretical and experimental approaches to study the antifouling properties of a new class of biomimetic polymers. When applied to the surface of an object, these polymers are anticipated to enhance the performance of medical devices by providing resistance to fouling by proteins, cells and bacteria.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB005772-03
Application #
7797671
Study Section
Biomaterials and Biointerfaces Study Section (BMBI)
Program Officer
Hunziker, Rosemarie
Project Start
2008-04-01
Project End
2012-03-31
Budget Start
2010-04-01
Budget End
2011-03-31
Support Year
3
Fiscal Year
2010
Total Cost
$378,603
Indirect Cost
Name
Northwestern University at Chicago
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
160079455
City
Evanston
State
IL
Country
United States
Zip Code
60201
Silies, Laura; Gonzalez Solveyra, Estefania; Szleifer, Igal et al. (2018) Insights into the Role of Counterions on Polyelectrolyte-Modified Nanopore Accessibility. Langmuir 34:5943-5953
Nap, Rikkert J; Gonzalez Solveyra, Estefania; Szleifer, Igal (2018) The interplay of nanointerface curvature and calcium binding in weak polyelectrolyte-coated nanoparticles. Biomater Sci 6:1048-1058
Ryu, Ji Hyun; Messersmith, Phillip B; Lee, Haeshin (2018) Polydopamine Surface Chemistry: A Decade of Discovery. ACS Appl Mater Interfaces 10:7523-7540
Morochnik, Simona; Nap, Rikkert J; Ameer, Guillermo A et al. (2017) Structural behavior of competitive temperature and pH-responsive tethered polymer layers. Soft Matter 13:6322-6331
Malaspina, David C; Longo, Gabriel; Szleifer, Igal (2017) Behavior of ligand binding assays with crowded surfaces: Molecular model of antigen capture by antibody-conjugated nanoparticles. PLoS One 12:e0185518
Zhou, Jiajing; Xiong, Qirong; Ma, Jielin et al. (2016) Polydopamine-Enabled Approach toward Tailored Plasmonic Nanogapped Nanoparticles: From Nanogap Engineering to Multifunctionality. ACS Nano 10:11066-11075
Solveyra, Estefania Gonzalez; Tagliazucchi, Mario; Szleifer, Igal (2016) Anisotropic surface functionalization of Au nanorods driven by molecular architecture and curvature effects. Faraday Discuss 191:351-372
Gonzalez Solveyra, Estefania; Szleifer, Igal (2016) What is the role of curvature on the properties of nanomaterials for biomedical applications? Wiley Interdiscip Rev Nanomed Nanobiotechnol 8:334-54
Zhou, Jiajing; Wang, Peng; Wang, Chenxu et al. (2015) Versatile Core-Shell Nanoparticle@Metal-Organic Framework Nanohybrids: Exploiting Mussel-Inspired Polydopamine for Tailored Structural Integration. ACS Nano 9:6951-60
Zelasko-Leon, Daria C; Fuentes, Christina M; Messersmith, Phillip B (2015) MUC1-Targeted Cancer Cell Photothermal Ablation Using Bioinspired Gold Nanorods. PLoS One 10:e0128756

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