Abstract

We propose to develop the use of plasmon-controlled fluorescence (PCF) and to apply this technology to the detection of cardiac markers. The effective use of PCF for sensing will depend on an understanding of the interactions of fluorophores with metallic nanostructures. These studies will provide the basis for using plasmon-controlled fluorescence (PCF) in medical diagnostics and biotechnology. This proposal evolved from our studies of the effects of random size metallic particles (silver island films, SIFs) and planar surfaces on nearby fluorophores. These studies showed that fluorophore-metal interactions result in modification of excitation and emission by enhancement of local incident fields and coupling of excited state energy into plasmons, which then radiate to the far field for detection.
Our Specific Aims are:
Specific Aim 1 : Nanofabrication of metallic nanostructures, including regular nanoparticle arrays, nanohole arrays and concentric nanorings.
Specific Aim 2. Determine the effects of these structures on nearby fluorophores, including intensities, lifetimes, photostability and directionality of the emission. We will also determine the effect of distance from the surface on these spectral properties.
Specific Aim 3. Correlate the near-field properties of these structures with their effects on fluorescence. Near-field properties of the structures will be determined experimentally using NSOM, which will be used to measure and image the evanescent field and light propagating away from the metal surfaces. These results will be compared with finite-difference time-domain (FDTD) calculations for the same structure.
Specific Aim 4. Use the metallic nanostructures for assays of the cardiac markers myoglobin, creatinine kinase (CK-MB), cardiac troponin l(cTnl) and B-type natriuretic peptide (BNB). For these analytes we will perform testing for specificity, sensitivity and reproducibility. The clinically relevant concentration of myogloblin are relatively high and this marker will be used for initial assay development. Cardiac troponin I and BMP are present at lower concentrations and their detection will drive development of nanostructures for high sensitivity detection. ? ? ?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB006521-02
Application #
7476385
Study Section
Biomedical Imaging Technology Study Section (BMIT)
Program Officer
Zhang, Yantian
Project Start
2007-08-01
Project End
2010-07-31
Budget Start
2008-08-01
Budget End
2009-07-31
Support Year
2
Fiscal Year
2008
Total Cost
$392,000
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Biochemistry
Type
Schools of Medicine
DUNS #
188435911
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

Badugu, Ramachandram; Jeng, Bennie H; Reece, E Albert et al. (2018) Contact lens to measure individual ion concentrations in tears and applications to dry eye disease. Anal Biochem 542:84-94
Zhang, Douguo; Xiang, Yifeng; Chen, Junxue et al. (2018) Extending the Propagation Distance of a Silver Nanowire Plasmonic Waveguide with a Dielectric Multilayer Substrate. Nano Lett 18:1152-1158
Zhang, Douguo; Zhu, Liangfu; Chen, Junxue et al. (2018) Conversion of isotropic fluorescence into a long-range non-diverging beam. Methods Appl Fluoresc 6:024003
Wang, Ruxue; Xia, Hongyan; Zhang, Douguo et al. (2017) Bloch surface waves confined in one dimension with a single polymeric nanofibre. Nat Commun 8:14330
Zhang, Douguo; Wang, Ruxue; Xiang, Yifeng et al. (2017) Silver Nanowires for Reconfigurable Bloch Surface Waves. ACS Nano 11:10446-10451
Zhu, Liangfu; Badugu, Ramachandram; Zhang, Douguo et al. (2017) Radiative decay engineering 8: Coupled emission microscopy for lens-free high-throughput fluorescence detection. Anal Biochem 531:20-36
Wang, Ruxue; Wang, Yong; Zhang, Douguo et al. (2017) Diffraction-Free Bloch Surface Waves. ACS Nano 11:5383-5390
Zhu, Liangfu; Zhang, Douguo; Wang, Ruxue et al. (2017) Out-of-Focal Plane Imaging by Leakage Radiation Microscopy. J Opt 19:
Zhu, Yu; Qiu, Dong; Yang, Guang et al. (2016) Selective and sensitive detection of MiRNA-21 based on gold-nanorod functionalized polydiacetylene microtube waveguide. Biosens Bioelectron 85:198-204
Xia, Hongyan; Wang, Ruxue; Liu, Yingying et al. (2016) Active Polymer Microfiber with Controlled Polarization Sensitivity. Adv Opt Mater 4:371-377

Showing the most recent 10 out of 97 publications