Embryonic stem cell (ESC) differentiation is a potentially powerful approach for generating a renewable source of cells for regenerative medicine. It is known that the microenvironment greatly influences ESC differentiation and self-renewal. Most biological studies have aimed in identifying individual molecules and signals. However, it is becoming increasingly accepted that the wide array of signals in the ESC microenvironment interact in a synergistic and antagonistic manner based on their temporal and spatial expression, dosage, and specific combinations. This interplay of microenvironmental factors regulates the ESC fate decisions to proliferate, self-renew, differentiate, and migrate. Despite this complexity, the study of stem cell cues in a systematic manner is technologically challenging, expensive, slow, and labor intensive. Here we propose to develop an enabling technology based on a high-throughput microfluidic system that overcomes many of these challenges. By providing a way of testing combinatorial microenvironments for directing stem cell differentiation, this approach promises to be of great benefit for tissue engineering.
A major limitation in treating cardiac injury is the failure of current therapies to induce myocardium regeneration. However, the source of cardiomyocytes for heart failure treatment remains an unsolved problem. We propose to use state-of-the-art microfabricated high-throughput microfluidic devices for studying growth and directed cardiac specific differentiation from embryonic stem cells.
Miri, Amir K; Khalilpour, Akbar; Cecen, Berivan et al. (2018) Multiscale bioprinting of vascularized models. Biomaterials : |
Saghazadeh, Saghi; Rinoldi, Chiara; Schot, Maik et al. (2018) Drug delivery systems and materials for wound healing applications. Adv Drug Deliv Rev 127:138-166 |
Leijten, Jeroen; Seo, Jungmok; Yue, Kan et al. (2017) Spatially and Temporally Controlled Hydrogels for Tissue Engineering. Mater Sci Eng R Rep 119:1-35 |
Bagherifard, Sara; Tamayol, Ali; Mostafalu, Pooria et al. (2016) Dermal Patch with Integrated Flexible Heater for on Demand Drug Delivery. Adv Healthc Mater 5:175-84 |
Shin, Su Ryon; Farzad, Raziyeh; Tamayol, Ali et al. (2016) A Bioactive Carbon Nanotube-Based Ink for Printing 2D and 3D Flexible Electronics. Adv Mater 28:3280-9 |
Rezaei Nejad, Hojatollah; Goli Malekabadi, Zahra; Kazemzadeh Narbat, Mehdi et al. (2016) Laterally Confined Microfluidic Patterning of Cells for Engineering Spatially Defined Vascularization. Small 12:5132-5139 |
Tamayol, Ali; Akbari, Mohsen; Zilberman, Yael et al. (2016) Flexible pH-Sensing Hydrogel Fibers for Epidermal Applications. Adv Healthc Mater 5:711-9 |
Akbari, Mohsen; Tamayol, Ali; Bagherifard, Sara et al. (2016) Textile Technologies and Tissue Engineering: A Path Toward Organ Weaving. Adv Healthc Mater 5:751-66 |
Shin, Su Ryon; Li, Yi-Chen; Jang, Hae Lin et al. (2016) Graphene-based materials for tissue engineering. Adv Drug Deliv Rev 105:255-274 |
Hjortnaes, Jesper; Goettsch, Claudia; Hutcheson, Joshua D et al. (2016) Simulation of early calcific aortic valve disease in a 3D platform: A role for myofibroblast differentiation. J Mol Cell Cardiol 94:13-20 |
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