This project is concerned with investigating: i) the diffusion into the tissues of the eye and of the orbit of electron opaque tracers introduced into the anterior chamber at controlled values of intraocular pressure; ii) the movement of electron opaque tracers from the vitreous body into the vessels of the retina and optic nerve. A necessary corollary of this study is the analyis with conventional electron microscopy of the morphology of the retinal vascular tree and a freeze-fracture analysis of the endothelial junctions; iii) the morphological aspects of the glaucoma induced by the introduction of alpha chymotrypsin into the posterior chamber. Animals of choice are rabbits and macaque monkeys. The precise knowledge of the anatomical mechanisms which regulate the intraocular pressure is necessary for a better understanding of the pathogenesis of glaucomas and for devising new pharmacological methods for their control.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY001349-12
Application #
3255924
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1979-06-01
Project End
1986-12-31
Budget Start
1986-06-01
Budget End
1986-12-31
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Boston University
Department
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118
Sagaties, M J; Raviola, G (1989) Immunogold staining of elastin in the wall of the retinal arteries in Macaca mulatta. Curr Eye Res 8:55-9
Raviola, G; Sagaties, M J; Miller, C (1987) Intercellular junctions between fibroblasts in connective tissues of the eye of macaque monkeys. A thin section and freeze fracture analysis. Invest Ophthalmol Vis Sci 28:834-41
Sagaties, M J; Raviola, G; Schaeffer, S et al. (1987) The structural basis of the inner blood-retina barrier in the eye of Macaca mulatta. Invest Ophthalmol Vis Sci 28:2000-14
Raviola, G (1986) Evidence for a secretory process, distinct from that of the aqueous humour, in the ciliary epithelium of Macaca mulatta. Trans Ophthalmol Soc U K 105 ( Pt 2):140-8
Smith, R L; Nishimura, Y; Raviola, G (1985) Interreceptor junction in the double cone of the chicken retina. J Submicrosc Cytol 17:183-6
Mwasi, L M; Raviola, G (1985) Morphology and permeability properties of blood capillaries in extraocular muscles of macaque monkeys. Graefes Arch Clin Exp Ophthalmol 223:9-15
Raviola, G; Butler, J M (1985) Asymmetric distribution of charged domains on the two fronts of the endothelium of iris blood vessels. Invest Ophthalmol Vis Sci 26:597-608
Raviola, G; Butler, J M (1985) Morphological evidence for the transfer of anionic macromolecules from the interior of the eye to the blood stream. Curr Eye Res 4:503-16