This proposal aims at elucidating functional and structural relationships between the retinal pigment epithelium (RPE) and photoreceptors which are necessary to the viability of the photoreceptors and are relevant to ocular disease. We will study adhesion between retina and RPE by separating the layers under a variety of experimental conditions, while monitoring the forces involved and the histologic consequences. We will study fluid transport across the RPE and the control of subretinal fluid volume by making experimental non-rhegmatogenous detachments in the living eye and correlating physiologic, fluorophotometric, histological, and electrophysiological data. We will also assess the importance of RPE-photoreceptor interactions by studying adhesion, transport and electrophysiology after removal of the photoreceptor outer segments by hemicholinium or tunicamycin. Our primary experimental animal for baseline studies will be the rabbit. Cats and monkeys will be used as more definitive models for the human eye. These experiments are relevant to a variety of human diseases in which the retina separates, subretinal fluid accumulates and visual function is lost. These studies may help suggest medical means of modifying subretinal fluid movement and retinal adhesion, to prevent and treat clinical detachments, and may help us to better understand retinal dystrophies and senile conditions which involve cellular loss at the level of the subretinal space.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY001678-11
Application #
3256109
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1981-08-01
Project End
1987-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
11
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305
Metlapally, Sangeetha; Tong, Jianliang L; Tahir, Humza J et al. (2016) Potential role for microfluctuations as a temporal directional cue to accommodation. J Vis 16:19
Metlapally, Sangeetha; Tong, Jianliang L; Tahir, Humza J et al. (2014) The impact of higher-order aberrations on the strength of directional signals produced by accommodative microfluctuations. J Vis 14:
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Takeuchi, A; Kricorian, G; Marmor, M F (1996) When vitreous enters the subretinal space. Implications for subretinal fluid protein. Retina 16:426-30
Chon, C H; Yao, X Y; Dalal, R et al. (1996) An experimental model of retinal pigment epithelial and neurosensory serous detachment. Retina 16:139-44
Chiang, R K; Yao, X Y; Takeuchi, A et al. (1995) Cytochalasin D reversibly weakens retinal adhesiveness. Curr Eye Res 14:1109-13
Takeuchi, A; Kricorian, G; Marmor, M F (1995) Albumin movement out of the subretinal space after experimental retinal detachment. Invest Ophthalmol Vis Sci 36:1298-305
Nayak, M S; Marmor, M F (1995) Post-ischemia ERG recovery is influenced by temperature. Curr Eye Res 14:81-5
Hageman, G S; Marmor, M F; Yao, X Y et al. (1995) The interphotoreceptor matrix mediates primate retinal adhesion. Arch Ophthalmol 113:655-60

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