The direction of this grant has been and will be to undertake clinical, physiologic, pathologic, histochemical and biochemical studies of human retinal diseases and to create comparable animal models so that pathologic, pathogenetic and therapeutic investigations may be explored. I. In the coming grant period, we shall evaluate the hypothesis, that vitamin C plays an important role in ameliorating photic injury of the retina as a superoxide radical scavenger. We propose to examine the distribution of reduced and oxidized ascorbates in the neural retina and RPE-choroid complex in normal guinea pig, monkey and surgically enucleated and autopsy human eyes. The pathologic alterations of the normal and scorbutic retinas after photic injury will be studied. The effect of vitamin C on its withdrawal on retinal photic injury will be studied. II. We shall explore the model of experimental disciform macular degeneration with sub-RPE neovascularization in monkey, developed after exposure to the light of an indirect ophthalmoscope. Repeated short light exposures will be given to stimulate the sub-RPE neovascularization. Systemic hypertension will be induced to examine the response of sub-RPE neovascularization to this vascular disturbance. III. We shall continue to obtain human eyes with drusen, senile choroidal sclerosis, senile macular degeneration, cystoid macular edema, diabetic retinopathy, sickle cell retinopathy and other retinopathies and choroidopathies for detail studies utilizing the latest clinical, physiologic, pathologic, histochemical and biochemical techniques so that preventive and therapeutic measures for our patients may be designed. In the investigation of the above human diseases and animal models, the clinical course will be studied by fundus photography and fluorescein angiography; the disruption of the blood-retinal barrier by vitreous fluorophotometry and horseradish peroxidase tracer technique; the pathologic changes by light and electron microscopy; the alterations of the interphotoreceptor matrix and cyclic-GMP phosphodiesterase by histochemical techniques, and the changes of the oxidized and reduced ascorbates in the neural retina and RPE-choroid complex by biochemical analysis.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY001903-10
Application #
3256309
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1979-03-01
Project End
1989-02-28
Budget Start
1985-03-01
Budget End
1986-02-28
Support Year
10
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Type
Schools of Medicine
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612
Lam, T T; Abler, A S; Tso, M O (1999) Apoptosis and caspases after ischemia-reperfusion injury in rat retina. Invest Ophthalmol Vis Sci 40:967-75
Takahashi, K; Lam, T T; Fu, J et al. (1997) The effect of high-dose methylprednisolone on laser-induced retinal injury in primates: an electron microscopic study. Graefes Arch Clin Exp Ophthalmol 235:723-32
Wang, M; Lam, T T; Tso, M O et al. (1997) Expression of a mutant opsin gene increases the susceptibility of the retina to light damage. Vis Neurosci 14:55-62
Al-Ubaidi, M R; Mangini, N J; Quiambao, A B et al. (1997) Unscheduled DNA replication precedes apoptosis of photoreceptors expressing SV40 T antigen. Exp Eye Res 64:573-85
Lam, T T; Siew, E; Chu, R et al. (1997) Ameliorative effect of MK-801 on retinal ischemia. J Ocul Pharmacol Ther 13:129-37
Li, S; Edward, D P; Ratnakar, K S et al. (1996) Clinicohistopathological findings of gelatinous droplike corneal dystrophy among Asians. Cornea 15:355-62
Abler, A S; Chang, C J; Ful, J et al. (1996) Photic injury triggers apoptosis of photoreceptor cells. Res Commun Mol Pathol Pharmacol 92:177-89
Buchi, E R; Kurosawa, A; Tso, M O (1996) Retinopathy in diabetic hypertensive monkeys: a pathologic study. Graefes Arch Clin Exp Ophthalmol 234:388-98
Li, S; Chang, C J; Abler, A S et al. (1996) A comparison of continuous versus intermittent light exposure on apoptosis. Curr Eye Res 15:914-22
Perlman, J I; McCole, S M; Pulluru, P et al. (1996) Disturbances in the distribution of neurotransmitters in the rat retina after ischemia. Curr Eye Res 15:589-96

Showing the most recent 10 out of 60 publications