Abstract

This is a proposal to study biochemistry and pharmacology of the retina with particular emphasis on cyclic nucleotides. The overall aim is to define the role of cyclic AMP in retinal function and metabolism. We will address and explore the hypothesis that retina contains cyclic AMP and cyclic GMP systems, each in different subcellular or cellular locations and each with different characteristics and functions. Utilizing quantitative histochemical methods and techniques we plan to identify, localize and characterize guanylate cyclases, adenylate cyclases, cyclic nucleotic phosphodiesterases and cyclic nucleotide-dependent protein kinases in retina. Regional purine nucleotide metabolism in retina also will be evaluated. In addition, we wish to define what factors (i.e., light, neurotransmitters, ions, cellular depolarization) regulate cyclic nucleotide levels in retina and explain their mechanisms. This project, which correlates retinal biochemistry and morphology, should help explain some molecular mechanisms underlying vision.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY002294-08
Application #
3256658
Study Section
(VID)
Project Start
1978-02-01
Project End
1986-03-31
Budget Start
1985-02-01
Budget End
1986-03-31
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Verhoeven, Virginie J M; Hysi, Pirro G; Wojciechowski, Robert et al. (2013) Genome-wide meta-analyses of multiancestry cohorts identify multiple new susceptibility loci for refractive error and myopia. Nat Genet 45:314-8
Hueter, R E; Murphy, C J; Howland, M et al. (2001) Refractive state and accommodation in the eyes of free-swimming versus restrained juvenile lemon sharks (Negaprion brevirostris). Vision Res 41:1885-9
McIntosh, H H; Blazynski, C (1994) Characterization and localization of adenosine A2 receptors in bovine rod outer segments. J Neurochem 62:992-7
Blazynski, C (1993) Characterization of adenosine A2 receptors in bovine retinal pigment epithelial membranes. Exp Eye Res 56:595-9
Blazynski, C; McIntosh, H (1993) Characterization of adenosine A2 receptors in bovine retinal membranes. Exp Eye Res 56:585-93
Blazynski, C; Woods, C; Mathews, G C (1992) Evidence for the action of endogenous adenosine in the rabbit retina: modulation of the light-evoked release of acetylcholine. J Neurochem 58:761-7
McIntosh, H; Blazynski, C (1992) Muscarinic receptor stimulated GTPase activity in synaptic membranes from bovine retina. J Neurochem 59:210-5
Woods, C L; Blazynski, C (1991) Characterization of adenosine A1-receptor binding sites in bovine retinal membranes. Exp Eye Res 53:325-31
Blazynski, C; Perez, M T (1991) Adenosine in vertebrate retina: localization, receptor characterization, and function. Cell Mol Neurobiol 11:463-84
McIntosh, H; Blazynski, C (1991) GAP-43-like immunoreactivity in the adult retina of several species. Brain Res 554:321-4

Showing the most recent 10 out of 22 publications