The purpose of this project is to study the recipient response to corneal homografts relined with heterologous corneal endothelium. The project uses the feline model of keratoplasty and investigates immune mediated phenomena in the local area of the transplant (aqueous humor of anterior chamber) as well as systemically. The experimental approach is to: (1) establish normal immune status values in the model, (2) thoroughly characterize the recipient immune system following successful corneal homotransplantation and following induced transplant rejection, and (3) then compare the recipient response to heterologous corneal endothelial cell lined homografts. Correlated methods of analysis include (1) clinical measurements (slit lamp biomicroscopy, specular corneal endothelial cell microscopy, and pachometry), (2) morphological studies (scanning and transmission electron microscopy, aqueous cell cytology), and (3) immunological studies (immunoglobulin concentrations, specific antibody titers to transplant related- and unrelated antigens, lymphocyte blastogenesis, cell mediated cytotoxicity, and antibody cell mediated cytotoxicity). Further studies include: (1) the role of corneal endothelium in evoking suppressor-like activity following successful corneal transplantation, (2) the value of mixed lymphocyte response studies in predicting the successful outcome of impending corneal transplantation, and (3) the practical use of other heterologous replacement endothelial cells in corneal transplantation.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY003573-07
Application #
3257938
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1981-02-01
Project End
1991-02-28
Budget Start
1987-12-01
Budget End
1991-02-28
Support Year
7
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
Schools of Medicine
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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