Abstract

Glycoproteins of bovine and human corneal stroma will be isolated, purified and characterized. In addition, the biosynthesis of glycoproteins in corneal stroma from normal donors and from patients with granular and macular dystrophies of the cornea and keratoconus will be investigated in organ culture as well as in tissue culture. With these techniques, it may be possible to identify specific glycoconjugate-related abnormalities, if present, in one or more of the above diseases. Growth characteristics of cat corneal endothelial cells in tissue culture will be investigated and plasma membrane glycoproteins of mitotic (rabbit) and nonmitotic (human and cat) corneal endothelial cells will be characterized and compared. Plasma membrane glycoproteins of cat and human corneal endothelial cells from young and old age groups will also be compared. Furthermore, the effect of lectins on rabbit corneal endothelial wound healing in organ culture and on DNA synthesis by rabbit corneal endothelial cells in tissue culture will be investigated. The role of plasma membrane glycoconjugates of corneal endothelial cells in mitosis and migration will be explored.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY004034-06
Application #
3258500
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1981-06-01
Project End
1988-06-30
Budget Start
1986-12-01
Budget End
1988-06-30
Support Year
6
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02111

  Publications

Panjwani, N; Zhao, Z; Baum, J et al. (1992) Acanthamoebae bind to glycolipids of rabbit corneal epithelium. Infect Immun 60:3460-3
Panjwani, N; Clark, B; Cohen, M et al. (1990) Differential binding of P. aeruginosa and S. aureus to corneal epithelium in culture. Invest Ophthalmol Vis Sci 31:696-701
Panjwani, N; Zaidi, T S; Gigstad, J E et al. (1990) Binding of Pseudomonas aeruginosa to neutral glycosphingolipids of rabbit corneal epithelium. Infect Immun 58:114-8
Panjwani, N; Michalopoulos, G; Song, J et al. (1990) Neutral glycolipids of migrating and nonmigrating rabbit corneal epithelium in organ and cell culture. Invest Ophthalmol Vis Sci 31:689-95
Panjwani, N; Drysdale, J; Clark, B et al. (1989) Protein-related abnormalities in keratoconus. Invest Ophthalmol Vis Sci 30:2481-7
Funderburgh, J L; Panjwani, N; Conrad, G W et al. (1989) Altered keratan sulfate epitopes in keratoconus. Invest Ophthalmol Vis Sci 30:2278-81
Panjwani, N; Baum, J (1988) A histochemical comparison of human corneal stromal glycoconjugates with eight other species. Distinct species-dictated differences in binding sites of Griffonia simplicifolia I. Histochemistry 89:41-5
Yue, B Y; Panjwani, N; Sugar, J et al. (1988) Glycoconjugate abnormalities in cultured keratoconus stromal cells. Arch Ophthalmol 106:1709-12
Panjwani, N; Rodrigues, M; Free, K et al. (1987) Lectin receptors of amyloid in corneas with lattice dystrophy. Arch Ophthalmol 105:688-91
Hsieh, P; Baum, J (1985) Effects of fibroblastic and endothelial extracellular matrices on corneal endothelial cells. Invest Ophthalmol Vis Sci 26:457-63