The long term objectives of this study are to investigate the factors responsible for the progressive layering of cells during development of the chick neural retina. Our working hypothesis is that cells possess and respond to unique cell surface characteristics which determine the position of cells within the tissue. In previous work, we have developed procedures that allow us to isolate specific cell populations from the 14 day embryonic neural retina. These cell populations possess distinct morphology and associative behavior. The immediate objectives of this project are to isolate and characterize factors which promote the adhesion of specific retinal cell types. The information obtained from this study will contribute to our understanding of the development of the complex neural structure of the retina, and, by extension, the central nervous system.
| Moyer, M; Bullrich, F; Sheffield, J B (1990) Emergence of flat cells from glia in stationary cultures of embryonic chick neural retina. In Vitro Cell Dev Biol 26:1073-8 |
| Sheffield, J B; Graff, D; Li, H P (1987) A solid-phase method for the quantitation of protein in the presence of sodium dodecyl sulfate and other interfering substances. Anal Biochem 166:49-54 |
| Li, H P; Sheffield, J B (1986) Retinal flat cells participate in the formation of fibers by retinal neuroblasts in vitro. Time lapse video studies. Invest Ophthalmol Vis Sci 27:307-15 |
| Li, H P; Sheffield, J B (1986) Retinal flat cells are a substrate that facilitates retinal neuron growth and fiber formation. Invest Ophthalmol Vis Sci 27:296-306 |
