Glycosyl transferases are enzymes involved in the synthesis of complex carbohydrates. They have been implicated in a variety of physiological processes from homeostasis to cell-cell adhesion. Embryonic chick neural retina cells have both an intracellular and a cell surface form of a beta-N-acetyl galactosaminyl transferase. Both forms exist as high density complexes with tightly bound acceptor. We propose to identify the transferase and its acceptor on two-dimensional electropherograms, analyze the mode through which the enzyme/acceptor is mobilized from its intracellular location to the cell surface, identify the components which comprise the high density complexes and determine whether the transferase/acceptor complex plays a role in cell adhesion.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
9R01EY005860-04A1
Application #
3261494
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1984-12-01
Project End
1987-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
Schools of Arts and Sciences
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715