Abstract

The objective is to determine the process affecting cellular repair of DNA and their effects on radiation responses of cells, particularly solar ultraviolet radiation. Attention is focussed on processes induced by radiation damage, and the factors which modify these cellualr reactions.
Specific aims i nvolve characterization of enhanced DNA repairs induced by UV light exposure in mammalian cells, regulation of the gene for DNA photolyase in E. coli (producing the enzyme responsible for photoenzymatic repair of pyrimidine dimers in DNA), and clonong of the photolyase gene from a marsupial cell.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM016547-21
Application #
3268959
Study Section
Radiation Study Section (RAD)
Project Start
1977-04-01
Project End
1989-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
21
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Texas-Dallas
Department
Type
Schools of Arts and Sciences
DUNS #
City
Richardson
State
TX
Country
United States
Zip Code
75080

  Publications

Alcorn, J L; Rupert, C S (1990) Regulation of photolyase in Escherichia coli K-12 during adenine deprivation. J Bacteriol 172:6885-91
Lorence, M C; Maika, S D; Rupert, C S (1990) Physical analysis of phr gene transcription in Escherichia coli K-12. J Bacteriol 172:6551-6
Sayed, N I; Rupert, C S (1989) Recovery of viral capacity in irradiated exponentially growing cells. Photochem Photobiol 50:153-6
Overberg, R; Chandrasena, G; Rupert, C S (1988) Radiation-induced recovery processes in cultured marsupial cells. Mutat Res 194:83-92