The understanding of how gene expression is controlled is fundamental to discovering the mechanisms involved in normal and abnormal cellular growth and differentiation in all biological systems. Recent information suggests that a large number of positive and negative regulatory factors are operative in controlling individual gene activity in bacteria as well as in multicellular organisms. it is becoming increasingly clear that the interplay of these regulatory components, rather than their individual activities, is critical to gene function. With this in mind we wish to continue our study of the factors involved in the control of the ilvBN and ompB operons of Escherichia coli. The goals of this work are twofold: First, to more thoroughly understand the interaction between integration host factor (IHF) and the ilvBN attenuation system. This work will involve in vivo and in vitro analysis of the transcriptional antitermination activity of IHF in the ilvBN leader. The second goal is to understand the role in ompB expression of a number of DNA binding proteins that interact with the ompB promoter region. A combined biochemical and genetic approach will be used in an attempt to determine the physiological significance and the precise molecular mechanisms involved in the interplay of these proteins in the regulation of ompB transcription.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM017152-22
Application #
3269065
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1977-09-01
Project End
1994-11-30
Budget Start
1990-12-01
Budget End
1991-11-30
Support Year
22
Fiscal Year
1991
Total Cost
Indirect Cost
Name
State University New York Stony Brook
Department
Type
Schools of Arts and Sciences
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794
Ramani, N; Hedeshian, M; Freundlich, M (1994) micF antisense RNA has a major role in osmoregulation of OmpF in Escherichia coli. J Bacteriol 176:5005-10
Sirko, A; Zehelein, E; Freundlich, M et al. (1993) Integration host factor is required for anaerobic pyruvate induction of pfl operon expression in Escherichia coli. J Bacteriol 175:5769-77
Huang, L; Tsui, P; Freundlich, M (1992) Positive and negative control of ompB transcription in Escherichia coli by cyclic AMP and the cyclic AMP receptor protein. J Bacteriol 174:664-70
Ramani, N; Huang, L; Freundlich, M (1992) In vitro interactions of integration host factor with the ompF promoter-regulatory region of Escherichia coli. Mol Gen Genet 231:248-55
Freundlich, M; Ramani, N; Mathew, E et al. (1992) The role of integration host factor in gene expression in Escherichia coli. Mol Microbiol 6:2557-63
Tsui, P; Huang, L; Freundlich, M (1991) Integration host factor binds specifically to multiple sites in the ompB promoter of Escherichia coli and inhibits transcription. J Bacteriol 173:5800-7
Huang, L; Tsui, P; Freundlich, M (1990) Integration host factor is a negative effector of in vivo and in vitro expression of ompC in Escherichia coli. J Bacteriol 172:5293-8
Tsui, P; Freundlich, M (1990) Integration host factor bends the DNA in the Escherichia coli ilvBN promoter region. Mol Gen Genet 223:349-52
Tsui, P; Freundlich, M (1988) Integration host factor binds specifically to sites in the ilvGMEDA operon in Escherichia coli. J Mol Biol 203:817-20
Tsui, P; Helu, V; Freundlich, M (1988) Altered osmoregulation of ompF in integration host factor mutants of Escherichia coli. J Bacteriol 170:4950-3

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