Formulated and executed at the level of genetic analysis, this project centers on developing a unified molecular mechanism that will account for all aspects of meiotic recombination in yeast. From methodological and programmatic viewpoints, the project centers on the characterization of correction deficient (cor) mutants, and the identification and analysis of other principal loci that influence formation and repair of heteroduplex DNA. Additionally, we are engaged in a comprehensive study of the ways in which autonomously replicating plasmid vectors, respectively carrying DNA inserts containing the wild type loci for arg4 and ade8+, are integrated into the disassociated from the normal yeast genome. arg4 and ade8 are among the most thoroughly understood loci in the test system. Numerous alleles have been identified and these include specific mutants distinguished by high basic conversion frequencies and equally high PMS frequencies. Such mutants will be studied exhaustively. Finally, we plan to study the interactions between cloned centromeres and mutants that affect chromosome disjunction. These mutants are identified by novel screening methods developed in this laboratory. The mutants affect meiotic disjunction and chromosome gain rather than loss is monitored. Their complete characterization and analysis is projected. In effect, we shall exploit and apply the existing strategies and technologies generated from the study of recombinant DNA to the investigation of meiotic recombination in Saccharomyces cerevisiae.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM017317-17
Application #
3269080
Study Section
Genetics Study Section (GEN)
Project Start
1976-12-01
Project End
1987-06-30
Budget Start
1986-05-01
Budget End
1987-06-30
Support Year
17
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of California Berkeley
Department
Type
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704