The goal of this research is to obtain metrical information from continuous wave and pulsed EPR spectroscopy of interacting spin systems in biological molecules. EPR transition energies, transition probabilities, and relaxation times will be calculated for spin systems of interest. These calculations will be calibrated with discrete metal complexes containing paramagnetic metals and nitroxyl spin labels. Exchange and dipolar contributions to the spin-spin interaction will be measured. The limits of applicability of various approaches to measuring spin-spin distances will be determined. These results will be applied to the nickel metalloenzyme urease, the copper-containing plastocyanin, and to CaATPase.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM021156-11
Application #
3270287
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1977-07-01
Project End
1989-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
11
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Denver
Department
Type
Schools of Arts and Sciences
DUNS #
City
Denver
State
CO
Country
United States
Zip Code
80208
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