Abstract

The three structural genes controlling incision of UV irradiation DNA (uvrA, uvrB, uvrC, and uvrAC) have been cloned into PBR322 and are being expressed in recA cells also carrying mutations in the uvrA, uvrB and uvrC genes, the gene products are being isolated as labelled proteins, their enzymatic properties determined as individual enzymes and when complemented for continued incising activities.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM022846-14
Application #
3271376
Study Section
Biochemistry Study Section (BIO)
Project Start
1978-09-01
Project End
1989-08-31
Budget Start
1988-09-01
Budget End
1989-08-31
Support Year
14
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
Schools of Public Health
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Baccarelli, A; Calista, D; Minghetti, P et al. (2004) XPD gene polymorphism and host characteristics in the association with cutaneous malignant melanoma risk. Br J Cancer 90:497-502
Qiao, Yawei; Spitz, Margaret R; Shen, Hongbing et al. (2002) Modulation of repair of ultraviolet damage in the host-cell reactivation assay by polymorphic XPC and XPD/ERCC2 genotypes. Carcinogenesis 23:295-9
Qiao, Yawei; Spitz, Margaret R; Guo, Zhaozheng et al. (2002) Rapid assessment of repair of ultraviolet DNA damage with a modified host-cell reactivation assay using a luciferase reporter gene and correlation with polymorphisms of DNA repair genes in normal human lymphocytes. Mutat Res 509:165-74
Landi, Maria Teresa; Baccarelli, Andrea; Tarone, Robert E et al. (2002) DNA repair, dysplastic nevi, and sunlight sensitivity in the development of cutaneous malignant melanoma. J Natl Cancer Inst 94:94-101
Grossman, L (2001) Nucleotide excision repair: Dick Setlow: how he influenced my scientific life. Environ Mol Mutagen 38:144-52
Hildebrand, E L; Grossman, L (1999) Oligomerization of the UvrB nucleotide excision repair protein of Escherichia coli. J Biol Chem 274:27885-90
Kovalsky, O I; Lin, C G; Grossman, L (1998) Selection of monoclonal antibodies for probing of functional intermediates in incision of UV-irradiated DNA by Uvr(A)BC endonuclease from Escherichia coli. Biochim Biophys Acta 1397:91-101
Kovalsky, O I; Grossman, L (1998) Accessibility of epitopes on UvrB protein in intermediates generated during incision of UV-irradiated DNA by the Escherichia coli Uvr(A)BC endonuclease. J Biol Chem 273:21009-14
Hildebrand, E L; Grossman, L (1998) Introduction of a tryptophan reporter group into the ATP binding motif of the Escherichia coli UvrB protein for the study of nucleotide binding and conformational dynamics. J Biol Chem 273:7818-27
ClGL; Kovalsky, O; Grossman, L (1998) Transcription coupled nucleotide excision repair by isolated Escherichia coli membrane-associated nucleoids. Nucleic Acids Res 26:1466-72

Showing the most recent 10 out of 45 publications