Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM023697-17
Application #
2174148
Study Section
Molecular and Cellular Biophysics Study Section (BBCA)
Project Start
1979-06-01
Project End
1998-06-30
Budget Start
1996-07-01
Budget End
1998-06-30
Support Year
17
Fiscal Year
1996
Total Cost
Indirect Cost

  Institution

Name
Colorado State University-Fort Collins
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
112617480
City
Fort Collins
State
CO
Country
United States
Zip Code
80523

  Publications

Griko, Y; Sreerama, N; Osumi-Davis, P et al. (2001) Thermal and urea-induced unfolding in T7 RNA polymerase: calorimetry, circular dichroism and fluorescence study. Protein Sci 10:845-53
Woody, A Y; Osumi-Davis, P A; Hiremath, M M et al. (1998) Pre-steady-state and steady-state kinetic studies on transcription initiation catalyzed by T7 RNA polymerase and its active-site mutants K631R and Y639F. Biochemistry 37:15958-64
Woody, A Y; Eaton, S S; Osumi-Davis, P A et al. (1996) Asp537 and Asp812 in bacteriophage T7 RNA polymerase as metal ion-binding sites studied by EPR, flow-dialysis, and transcription. Biochemistry 35:144-52
Lopez de Saro, F J; Woody, A Y; Helmann, J D (1995) Structural analysis of the Bacillus subtilis delta factor: a protein polyanion which displaces RNA from RNA polymerase. J Mol Biol 252:189-202
Osumi-Davis, P A; Sreerama, N; Volkin, D B et al. (1994) Bacteriophage T7 RNA polymerase and its active-site mutants. Kinetic, spectroscopic and calorimetric characterization. J Mol Biol 237:5-19
Osumi-Davis, P A; de Aguilera, M C; Woody, R W et al. (1992) Asp537, Asp812 are essential and Lys631, His811 are catalytically significant in bacteriophage T7 RNA polymerase activity. J Mol Biol 226:37-45
Knoll, D A; Woody, R W; Woody, A Y (1992) Mapping of the active site of T7 RNA polymerase with 8-azidoATP. Biochim Biophys Acta 1121:252-60
Shimer Jr, G H; Woody, A Y; Woody, R W (1988) Spectroscopic analysis of DNA base-pair opening by Escherichia coli RNA polymerase. Temperature and ionic strength effects. Biochim Biophys Acta 950:354-65
Woody, A Y; Evans, R K; Woody, R W (1988) Characterization of a photoaffinity analog of UTP, 5-azido-UTP for analysis of the substrate binding site on E. coli RNA polymerase. Biochem Biophys Res Commun 150:917-24
Wheeler, A R; Woody, A Y; Woody, R W (1987) Salt-dependent binding of Escherichia coli RNA polymerase to DNA and specific transcription by the core enzyme and holoenzyme. Biochemistry 26:3322-30

Showing the most recent 10 out of 13 publications