Abstract

The purpose of this study is to monitor the structure of cell junctions (nexus or gap junction) under various conditions and relate that information to the conductance and permeability of the structure. The parameters to be manipulated are intracellular pH, temperature and solvents (D2O vs. H2O). Hopefully experiments like these will illuminate the suspected intercellular channel.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM024905-06
Application #
3272640
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1983-07-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
State University New York Stony Brook
Department
Type
Schools of Medicine
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794

  Publications

Brink, P R; Fan, S F (1989) Patch clamp recordings from membranes which contain gap junction channels. Biophys J 56:579-93
Brink, P R; Mathias, R T; Jaslove, S W et al. (1988) Steady-state current flow through gap junctions. Effects on intracellular ion concentrations and fluid movement. Biophys J 53:795-807
Graber, M; Brink, P R; DiLillo, D et al. (1987) Permeabilizing the granular cell of toad and turtle bladder: lack of cell coupling. Am J Physiol 253:F588-94
Verselis, V; Brink, P R (1986) The gap junction channel. Its aqueous nature as indicated by deuterium oxide effects. Biophys J 50:1003-7